同位素稀释-电感耦合“冷”等离子体质谱法准确测定人血清中钾、钙、镁的含量

Accurate Determination of K,Ca and Mg in Human Serum by Isotope Dilution Inductively Coupled“Cold”Plasma Mass Spectrometry

血清电解质是骨骼和细胞健康的重要标志物,常通过分析血清中K、Ca、Mg和Na含量来监测其浓度水平的变化。本研究采用同位素稀释-电感耦合等离子体质谱法(ID-ICP-MS),结合"冷"等离子体和碰撞反应池两种模式,加入^(41)K、^(42)Ca和^(25)Mg 3种浓缩同位素稀释剂,同时测定了K、Ca和Mg含量。该方法省去了样品前处理过程中繁琐的基体分离步骤,消除了质谱测量过程中Ar基分子离子的干扰,实现了人血清中K、Ca和Mg含量的准确测定。通过测定NIST SRM956c冷冻人血清无机成分,分析标准物质中K、Ca和Mg含量,来验证该方法的可行性和准确度,测得的结果与标准值一致。本方法可用于临床研究中元素含量分析,也可用于生物基体标准物质的定值。

Electrolytes in serum are important biomarkers for skeletal and cellular health.The levels of electrolytes are monitored by measuring the K,Ca,Mg and Na in blood serum.An accurate method for the determination of K,Ca and Mg in serum by taking advantage of the ＂cold＂plasma mode and collision reaction cell of the isotope dilution-inductively coupled plasma mass spectrometry（ID-ICP-MS）was developed.The three kinds isotope of 41K,42Ca and 25Mg were added at the same time,while the contents of K,Ca and Mg were also measured.The proposed method can eliminate the need for a slow and tedious sample digestion procedure,and also can avoid the molecular ions interference.The national institute of standards and technology standard referencematerial 956 cinorganic components in frozen human serum was analyzed by the method,and the measured elements values were in good accord with the standard values.