摘要
试验旨在研究在海南夏季高温饲养条件下,山羊补饲硒和维生素E对其精液品质、抗氧化酶活性及热休克蛋白表达的影响。选用16只健康状况良好、体重相近的海南黑山羊成年公羊,随机分成4组,分别饲喂基础日粮(对照组)、基础日粮+0.5mg/kg Se(Se组)、基础日粮+100mg/kg VE(VE组)和基础日粮+0.5mg/kg Se+100mg/kg VE(Se+VE组),试验期93d。试验期结束前1周采集精液,评价精液品质、测定精浆抗氧化酶活性和精液热休克蛋白表达。结果表明,与对照组相比,补饲Se和VE对海南黑山羊射精量影响差异不显著(P>0.05);补饲Se和VE能显著提高海南黑山羊的精子密度和精子活力(P<0.05),极显著降低精子畸形率(P<0.01);极显著增加精浆谷胱甘肽过氧化物酶活性和总抗氧化能力(P<0.01),显著增加超氧化物歧化酶和过氧化氢酶活性(P<0.05),显著降低丙二醛浓度(P<0.05);补饲Se和VE还极显著降低了精液HSP70和HSP90mRNA的表达水平(P<0.01),但各补饲组之间对精液品质改善效果和热休克蛋白表达丰度的影响存在一定差异。综上所述,补饲Se和VE有助于提高热带地区夏季高温季节山羊精子密度、精子活力,降低畸形率,同时还能增强精浆中抗氧化酶的活性,改善精液品质,进而起到缓解环境热应激的效果。
This experiment was conducted to study the effect of selenium and vitamin E supplement on semen quality,antioxidant enzyme activities and heat shock protein expression of goat in Hainan high temperature season.16 adult Hainan Black goat with good health and approximate weight were randomly divided into 4groups,fed with basal diet(control group),basal diet+0.5mg/kg Se(Se group),basal diet+100mg/kg VE(VE group),and basal diet+0.5mg/kg Se+100mg/kg VE(Se+VE group),respectively.The experimental period was 93 d.Semen samples were collected in the last week of the experiment on two consecutive days.The semen quality,antioxidant enzyme activities and heat shock protein expression were analyzed.The results showed that compared with control group,the ejaculate volume was not significantly affected by Se or VE supplement(P〈0.05).Sperm density and sperm motility were increased significantly by Se and VE supplement(P〈0.05),and the abnormal rate was decreased extremely significant-ly(P〈0.01).The goats fed with Se and VE also had higher activities of GSH-Px(P〈0.01),SOD(P〈0.05),CAT(P〈0.05)and T-AOC(P〈0.01),and lower MDA concentration in seminal plasma(P〈0.05).The relative expression levels of HSP70 and HSP90mRNA in supplement groups were decreased extremely significantly(P〈0.01).However,there were some certain differences between the Se and VE supplement groups on semen quality and heat shock protein expression.In conclusion,the supplementation of Se and VE could help to improve goat semen quality by increasing the sperm density,sperm motility,the antioxidant enzyme activities,and decreasing the abnormal rate in hot season of Hainan.Finally,Se and VE supplement had good effects on relieving the environment heat stress.
出处
《中国畜牧兽医》
CAS
北大核心
2016年第1期101-107,共7页
China Animal Husbandry & Veterinary Medicine
基金
中央级公益性科研院所基本科研业务费专项资金(中国热带农业科学院热带作物品种资源研究所)(1630032014024)
农业财政项目(2015)"海南省雷州山羊种质资源保护与高效利用"
关键词
精液品质
抗氧化酶
热休克蛋白
硒
维生素E
semen quality
antioxidant enzyme
heat shock protein
selenium
VE