紫菀不同极性段提取物对SD大鼠亚慢性毒性试验研究

Study on Subchronic Toxicity Test of Different Polarity Section Extracts fromAster tataricus L.f.on SD rats

本试验旨在观察紫菀不同极性段提取物对SD大鼠的肝脏毒性损伤。SD大鼠随机分为空白组、石油醚组、乙酸乙酯组、正丁醇组、母液组、75%乙醇组,每组10只,灌胃给药0.34g生药/kg体重,连续28d,观察对大鼠摄食量、体增重、脏器系数、尿液指标、血常规、血清生化指标、肝脏组织抗氧化酶、病理切片等的影响。试验结果显示各组大鼠体增重、肝脏组织抗氧化酶、尿液指标、脏器系数均无显著变化(P>0.05);雄鼠乙酸乙酯组、母液组白细胞计数(WBC)显著降低(P<0.05),正丁醇组极显著降低(P<0.01),雌鼠石油醚组、乙酸乙酯组WBC极显著降低(P<0.01),正丁醇组显著降低(P<0.05);雌鼠乙酸乙酯组乳酸脱氢酶(LDH)显著升高(P<0.05);病理组织学检查可见石油醚组和乙酸乙酯组大鼠肝脏出现轻微的肝索紊乱、颗粒变性,其余组与空白组相比无明显差异。结果表明在0.34g生药/kg体重给药条件下,紫菀的石油醚和乙酸乙酯提取物对大鼠产生轻微的肝脏毒性。

The aim of the experiment was to observe the liver toxicity of different polarity section extracts from Aster tataricus L.f.on SD rats.The subchronic test was performed via the daily oral administration of Aster tataricus L.f.at dose of 0.34g/kg body weight in SD rats which were divided into six groups（control group,petroleum ether group,ethyl acetate group,N-butyl alcohol group,mother liquid group and 75% ethanol group）,observing the effects on food consumption,body weight gain,viscera coefficient,urine examination,blood routine examination,serum biochemistry indices,liver antioxidant function analysis and histopathological observation.There was no significant changes of body weight gain,viscera coefficient,urine examination and liver antioxidant function among six groups（P〈0.05）,but WBC significantly and extremely significantly decreased in male rats of ethyl acetate group,mother liquid group and N-butyl alcohol group（P〈0.05;P〈0.01）,and significantly and extremely significantly decreased in female ratsof N-butyl alcohol group and petroleum ether group,ethyl acetate group（P〈0.05;P〈0.01）;The LDH significantly increased in female rats of ethyl acetate group（P〈0.05）;Slight congestion and necrosis were showed in liver in petroleum ether group and ethyl acetate group,there was no differences observed in other groups.The extracts of Aster tataricus L.f.,especially petroleum ether extract and ethyl acetate extract could cause slight liver toxicity under the dose of 0.34g/kg body weight.