小鼠骨髓间充质干细胞SCA-1^+/CD45^+/CD31^+亚群归巢基因研究

Genetic research of mouse bone marrow mesenchymal stem cells subsets SCA-1^+/CD45^+/CD31^+ homing ability

目的:探讨小鼠骨髓间充质干细胞(mBMSCs)亚群SCA-1+/CD45+/CD31+归巢分子基础。方法:以小鼠心脏干细胞表面分化抗原检测mBMSCs后,以CD45、CD31为标准分选得到4个亚群。体外将4个亚群采用transwell小室,检测各组的归巢能力。分别将各亚群细胞注入心肌梗死48h模型。小鼠体内注射后48h、96h、7d处死小鼠取其心脏,完成小动物活体成像并检测其荧光强度。可见SCA-1+/CD45+/CD31+组归巢能力优于其他各组。而后采用基因芯片完成Agilent小鼠全基因4×44K芯片,从分子水平对SCA-1+/CD45+/CD31+亚群归巢能力进行探讨。结果:将SCA-1+/CD45+/CD31+亚群与其他亚群有关归巢基因的聚类分析及Network分析的结果比较可见,Dcx及MADCAM1基因为两结果交集,是有关归巢的关键基因。结论:mBMSCs为一多克隆的细胞群体。SCA-1+/CD45+/CD31+亚群归巢能力方面优于其他亚群;其分子机制在于Dcx及MADCAM1基因表达较其他亚组多见。

Objective：To explore the molecular basis of the homing ability of the SCA-1^＋/CD45^＋/CD31^＋ subgroup of mBMSCs.Method：The mBMSCs were detected using mouse cardiac stem cell surface differentiation antigen,and four subgroups of mBMSCs were separated based on CD45 and CD31.The homing ability of the four subgroups was evaluated in vitro using transwell migration assay.The mBMSCs of different subgroups were injected into mice suffering from myocardial infarction for 48 h.The mice were sacrificed 48 h,96h,and 7days after the injection,and their hearts were analyzed by whole-body imaging and fluorescence spectroscopy.Result：It＇s showed that the SCA-1^＋/CD45^＋/CD31^＋ subgroup had the strongest homing ability among the subgroups.Moreover,the homing ability of the SCA-1^＋/CD45^＋/CD31^＋ subgroup was explored on a molecular level by using Agilent Whole Mouse Genome Oligo Microarray（4×44K）.Cluster and network analyses of the homing gene of SCA-1^＋/CD45^＋/CD31^＋ subgroup and other subgroups showed that Dcx and MADCAMI genes were the key genes related to homing.Conclusion：The mBMSCs were the polyclone cell group.The homing ability of the SCA-1^＋/CD45^＋/CD31^＋ subgroup was superior to that of the other subgroups.In addition,the molecular mechanism of the SCA-1^＋/CD45^＋/CD31^＋ subgroup in relation to Dcx and MADCAMI expression was more frequently observed than that of the other subgroups.