转MvP5CS基因棉花抗旱性及其育种价值评价

Analysis of Drought Resistance and Breeding Value Evaluation of Trans-MvP5CS Gene Cotton

利用花粉管通道法,获得的转Δ1-二氢吡咯-5-羧酸合成酶(Pyrroline-5-carboxylate synthetase,MvP5CS)高代棉花材料,比较田间干旱胁迫下转MvP5CS基因的T6代3个株系植株与受体棉花材料D5主要生理生化指标和农艺性状的差异。结果表明:从生理生化指标分析,转基因棉花比非转基因棉花植株更有利于在干旱胁迫条件下生长。从干旱胁迫后测量的农艺性状、产量性状和纤维品质的比较发现,转基因棉花植株的叶片颜色更绿;植株的主根更长,须根更多,更强壮;转基因棉花在单铃重、籽棉产量、皮棉产量、棉花衣分等性状明显优于非转基因棉花;纤维长度和强度略有增加,马克隆值降低,品质得到改善。

Using the method of pollen tube pathway,the high generation transferred MvP5CS（ Pyrroline-5-carboxylate synthetase,MvP5CS） cotton material has been abtained. Comparing the main difference between the physiological and biochemical and difference in agronomic traits between the transfer Mv P5CST6-generation cotton and non-genetically modified cotton under drought stress. The results show that： the transgenic cotton plants are more conducive to growth in the drought stress than non-transgenic cotton from analyzing the physiological and biochemical. After drought stress measurement of agronomic traits,yield traits and fiber quality： the color of the transgenic cotton leaves is greener and have longer taproot,stronger fibrous root. The transgenic cotton plants are significantly superior to the non-transgenic cotton in single boll weight,seed cotton yield,lint yield,fiber yield and other traits. Fiber length and strength increased slightly,incompatible micronaire is reduced,and quality gets improvement. This three transgenic cotton lines could provide new materials for the cotton resistance breeding.