桔梗糖蛋白的组成分析和对·OH的清除作用的研究

Study on the composition analysis and inhibition of·OH of glycoprotein from Platycodon grandiflorum

分离纯化经超临界CO_2辅助提取的桔梗糖蛋白,确定其单糖及氨基酸组成,考察其对·OH自由基的清除作用。以DEAE-52纤维素柱及Sephadex G-75柱分离纯化桔梗糖蛋白,分别利用PMP法柱前衍生和OPA-FMOC联合衍生测定糖蛋白中单糖和氨基酸的种类与组成,分光光度法检测桔梗糖蛋白对·OH自由基的清除作用。结果显示:桔梗糖蛋白主要由7种单糖及15种氨基酸组成,平均糖含量为61.82%,蛋白含量为33.11%,对·OH的清除能力的IC50值为0.99 mg/m L。由此表明:桔梗糖蛋白具有一定的抗氧化能力,为其用于功能食品、药物制造等方面发展提供理论依据。

Separation and purification of glycoprotein which were extracted by super critical CO_2 from Platycodon grandiflorum.Then the monosaccharide and amino acids composition and the inhibition of ·OH of glycoprotein were investigated. The glycoprotein were extracted from Platycodon grandiflorum and purified by DEAE- 52 cellulose column and Sephadex G-75 gel column.Monosaccharide and amino acids of glycoprotein were analyzed by pre- column derivatization with PMP and OPA- FMOC respectively. The results showed that Platycodon grandiflorum glycoprotein was composed of 7 kinds of monosaccharide（ average content of 61.82%） and 15 kinds of amino acids（ average content of 33.11%）. IC_（50） value of inhibition ·OH was 0.99 mg / m L. The study showed that·OH could be inhibited by the Platycodon grandiflorum glycoprotein effectively,which provided the theoretical basis for the further study on functional food and the drugs manufacture.