青荚叶多酚超声波辅助提取工艺及抗氧化活性研究

Study on the ultrasound- assisted extraction and antioxidant capacity of Polyphenols from Helwingia Japonica

确定超声波辅助提取青荚叶多酚的最佳工艺条件及其体外抗氧化活性。在单因素实验的基础上,固定超声功率65 W,提取次数2次,通过响应面法优化设计提取多酚的工艺参数,并对其抗氧化活性进行测定分析。结果表明:超声辅助提取青荚叶多酚的最佳工艺参数为乙醇体积分数40.8%、提取时间4.8 min、料液比1∶60(g∶m L)、提取温度67.5℃,多酚提取量为29.43 mg/g。青荚叶多酚具有较强的还原能力,清除DPPH自由基和ABTS+·自由基IC50分别为44.28、35.07 mg/L。因此,青荚叶可作为潜在天然抗氧化剂应用于食品和医药工业中。

The research aimed to determine the optimal ultrasonic- assisted extraction process of Helwingia japonica helwpolyphenols and its antioxidant activity in vitro. On the basis of design and analysis of single- factor experiments,through which the optimal ultrasonic power and the frequency of extraction were found to be 65 W and two times,the technological conditions of ultrasonic- assisted for the Helwingia japonica polyphenols were optimized by response surface methodology and the antioxidant activity of Helwingia japonica polyphenols was also studied by DPPH· and ABTS~＋·.The result showed that the optimum extraction conditions of Helwingia japonica polyphenols were the volume fraction of 40.8% ethanol,ultrasonic time 4.8 min,solid- liquid ratio 1 ∶ 60（ g∶ m L）,extraction temperature 67.5 ℃.Under this condition,the yield of polyphenols obtained was 29.43 mg /g.The tests of antioxidant capacity showed that Helwingia japonica polyphenols had strong deoxidizing ability,and the IC_（50） of DPPH· and ABTS~＋·were 44.28 mg /L and 35.07 mg /L respectively.Therefore,Helwingia japonica has a good potential as a natural antioxidant used in food or medicine industries.