不同品种南瓜多糖含量测定及比较

Testing and Comparison of the Polysaccharide Content for Different Species of Pumpkins

[目的]测定并比较不同品种南瓜多糖含量,筛选出多糖含量最高的品种。[方法]采用无水乙醇去除南瓜色素,通过水提法获得南瓜多糖溶液,蒽酮-硫酸比色法测定34个品种南瓜中多糖含量,比较各品种南瓜多糖含量的差异性。[结果]试验表明,蒽酮-硫酸法测定南瓜多糖含量的精密度试验RSD值为1.35%,稳定性试验RSD值为2.24%,加样回收率平均值为100.41%、RSD值为1.51%。分析得出不同品种南瓜的多糖含量差异较大,其中中国南瓜♂的多糖含量最高,为74.11 mg/g;JHX-20的多糖含量最低,为13.42mg/g。[结论]研究可为南瓜新品种开发和培育提供有效的科学依据,为南瓜多糖产品的开发利用奠定基础。

[Objective]To test and compare polysaccharide content in different species of pumpkins,pick out the variety with the highest polysaccharide. [Method] Pigments in pumpkins were removed by absolute ethyl alcohol. The polysaccharide solutions were obtained by water extract method. Then,polysaccharide contents in 34 species of pumpkins were measured by anthrone-sulfuric acid colorimetric method. A comparison was made to analyze the difference of the polysaccharide contents in each species of pumpkins. [Result] From the tests by adopting anthrone-sulfuric acid colorimetric method,it was observed that the RSD value of precision test was 1. 35% and that of the stability test was 2. 24%; the average recovery rate of added samples was 100. 41% and the corresponding RSD value was 1. 51%. By comparing and analyzing the test results,it was indicated that various pumpkins differ considerably in their polysaccharide contents,among which that of the China＇s pumpkin ♂ has the maximum value of 74. 11 mg / g and that of the JHX-20 has the minimum value of 13. 42 mg / g. [Conclusion] The study can provide effective scientific basis for the development and culture of new species of pumpkin,and also lay a foundation for the development and utilization of the pumpkin polysaccharide products.