电颈针干预对急性脑出血大鼠脑组织水通道蛋白4表达及脑含水量的影响

Effects of Neck Electrical Acupuncture on Brain Water Content and Aquaporin4 Expression in Intracerebral Hemorrhage Rats

目的研究电颈针干预对急性脑出血大鼠脑组织水通道蛋白4(AQP4)表达、脑含水量等的影响,探讨电颈针对急性期脑出血后脑水肿的治疗作用。方法研究将84只成年SD大鼠随机分为假手术组,模型组,电颈针组;模型组和电颈针组依据干预时间分别设1、3、5 d 3个亚组,对相应组进行脑组织含水量、AQP4含量和m RNA含量检测。结果模型组与电颈针各组AQP4蛋白含量均高于假手术组,且电颈针组较模型组减低。电颈针3 d组AQP4 m RNA表达低于模型3 d组,且均高于假手术组。模型与电颈针各组大鼠脑组织含水量均高于假手术组(P<0.01),电颈针组各亚组脑组织含水量均低于模型组(P<0.05)。结论电颈针能显著降低脑出血大鼠AQP4蛋白和基因表达、脑组织含水量,可以抑制脑出血后脑水肿,起到神经保护作用。

Objective： To investigate the effects of neck electrical acupuncture on brain water content and AQP4 expression in intracerebra[ hemorrhage rats. Methods： 84 SD rats were randomly divided into three groups： the sham operated group,the model group and neck electrical acupuncture group. The model group and neck electri- cal acupuneture group were divided into three subgroups： one-day subgroup, three-day subgroup and five-day subgroup. The brain water content,the content of AQP4 and mRNA in the corresponding group were detected. Results： The content of AQP4 protein higher in model group and neck electrical acupuncture group than in the sham operated group, and the the content of AQP4 protein was lower in neck electrical acupuncture group than in the model group. Compared with those in the sham group,the expression of AQP4 mRNA and AQP4 protein in- creased significantly in the model group （P〈 0.01 ）,and the brain water content showed the same trend. Conclu- sion： Neck electrical acupuncture can significantly restrain the AQP4 expression to reduce the content of AQP4 protein and alleviate cerebral edema after intracranial hemorrhage in rats so that to protect brain tissue.