胡黄连苷Ⅱ对大鼠脑缺血再灌流损伤的保护作用及对AQP4、MMP-9表达的影响

Protective Effect of Picroside Ⅱ on Cerebral Ischemia Reperfusion Injury in Rats and the Expression of MMP-9 and AQP4

目的观察胡黄连苷Ⅱ对大鼠脑缺血再灌流损伤的保护作用及对AQP4、MMP-9表达的影响。方法选取120只成年健康雄性大鼠,随机选择30只作为假手术组,其余90只制备脑缺血再灌注模型,成功78只,平均分为模型组和胡黄连苷Ⅱ组。胡黄连苷Ⅱ组给予胡黄连苷Ⅱ腹腔注射,模型组、假手术组给予等量的生理盐水腹腔注射,假手术组不制备脑缺血模型,其他步骤均同模型组。检测3组大鼠神经功能缺失评分、DCI、ACI、CIV,以及水通道蛋白4(AQP4)、基质金属蛋白酶9(MMP-9)和环氧合酶2(COX2)表达水平。结果胡黄连苷Ⅱ组神经功能缺失评分、DCI、ACI、CIV水平较假手术组显著降低,模型组各水平也较假手术组明显降低,且胡黄连苷Ⅱ组较模型组也明显降低;胡黄连苷Ⅱ组的AQP4、MMP-9和COX2表达水平分别为(1.12±0.14)、(0.95±0.08)、(0.58±0.05)ng/m L,较模型组明显降低,较假手术组显著升高,差异均具有统计学意义(均P<0.05)。结论胡黄连苷Ⅱ可以显著抑制AQP4、MMP-9表达,对脑缺血再灌流损伤具有一定的保护作用。

Objective： To explore and study the protective effect of picroside II on cerebral ischemia reperfusion injury in rats and the expression of MMP-9 and AQP4. Methods： 30 rats were randomly selected from 120 adult healthy male ones as normal control group,the remaining 90 of preparing cerebral ischemia reperfusion model.78 successful rats were divided into the observation group and the solvent group. The observation group received picroside II by intraperitoneal injection,while the solvent control group were given the same amount of saline by intraperitoneal injection,and the normal control group were not prepared for the rat model of focal cerebral ischemia,but other steps were the same as the solvent group. The neurological function score,DCI,CIV,ACI,AQP4,MMP-9 and COX2 expression in the three groups were observed and compared. Results： In the observation group,neurological deficit score,DCI,ACI,CIV levels decreased significantly compared with the normal control group,and each level of the solvent group were lower than those in the normal control group,and the data of the observation group compared with those of the solvent group decreased significantly. AQP4,MMP-9 and COX2 expression level of the observation group were respectively（1.12±0.14）,（0.95±0.08）,（0.58±0.05） ng/m L,compared with the solven group,decreasing obviously,and compared with the normal control group,increasing significantly.Differences were statistically significant（P 0.05）. Conclusion： Picroside II can inhibit the expression of AQP4 and MMP-9,which has protective effects on cerebral ischemia reperfusion injury.