LOX-1和炎症因子在糖尿病肾病中的作用及糖肾颗粒早期干预研究

The role of LOX-1 and inflammatory cytokines in diabetic nephropathy and early intervention research Tangshen particles

目的:探讨LOX-1和炎症因子在糖尿病肾病中的作用及其机制。方法:SD大鼠随机分为正常对照组、糖尿病模型组。以高脂高糖饮食加低剂量注射链脲佐菌素复制2型糖尿病大鼠模型。动态观察:生化指标和肾脏病理变化,LOX-1和TGFβ1蛋白和m RNA表达,检测血清MCP-1、ICAM和TNFα的浓度。结果:随着造模时间的延长,模型各组血清TC、TG、LDL含量和24h尿蛋白量逐渐升高;MCP-1、ICAM和TNFα水平均增高;LOX1和TGFβ1蛋白和m RNA表达逐渐增加;糖肾颗粒组指标降低,和模型组比较有显著差异。结论:糖肾颗粒可降低LOX-1和TGFβ1的表达,降低炎症因子的释放,提示糖肾颗粒可能是通过抑制LOX-1和TGFβ1表达进而抑制炎症反应从而起到保护肾脏的作用,提示LOX-l在DN发生和发展过程中起重要作用。

Objective： To investigate the LOX-1 and inflammatory factors and its mechanism in diabetic nephropathy. Methods： SD rats were randomly divided into normal control group, diabetic model group. High-fat high-sugar diet plus low-dose injection of streptozotocin copy type 2 diabetic rat model. Orbit： biochemical parameters and renal pathological changes, LOX 1-TGFβ1 protein and mRNA expression and to detect serum concentrations of MCP-1, ICAM and TNFα in. Results： With the extension of modeling time, the model of each group serum TC, TG, LDL and 24h urine protein content gradually increased; MCP-1, ICAM and TNFα levels were elevated; LOX1 and TGFβ1 protein and mRNA expression increased gradually; Tangshen particle group index lower, and the model group were significantly different. Conclusion： Tangshen particles can reduce the expression of LOX-1 and TGFβ1, reducing the release of inflammatory cytokines, suggesting Tangshen particles probably by inhibiting the expression of LOX-1 and TGFβ1 thereby inhibiting the inflammatory response and thus play a role in protecting the kidney, suggesting LOX- 1 play an important role in the onset and development of DN.