微小RNA-31靶向作用Rasp21蛋白活化子1对结肠癌细胞增殖和迁移的影响

The influence of microRNA-31 on cell proliferation and migration of colorectal cancer cell by targeting Ras p21 protein activator 1

目的观察微小RNA（miRNA，miR）-31对结肠癌细胞增殖和迁移的影响，并探讨其作用机制。方法采用反转录聚合酶链反应（RT—PCR）的方法检测miR-31在结肠癌细胞株和结直肠癌患者癌组织中的表达，细胞计数试剂盒（CCK-8）法和细胞划痕实验分别检测过表达miR-31后结肠癌细胞增殖和迁移，Western blot检测过表达miR-31的结肠癌细胞中Rasp21蛋白活化子1（RASA1）的表达。结果miR-31在结肠癌组织和细胞中高表达（P〈0．05），过表达miR-31可促进结肠癌细胞的增殖和迁移能力。同时，转染miR-31模拟物组RASA1的相对表达量为0．21±0．05，miR-31抑制剂组的相对表达量为2．464-0．35，两者比较差异有统计学意义（P〈0．05）。结论miR-31对结肠癌细胞的增殖和迁移具有促进作用，其机制与靶向作用于RASA1蛋白有关。

Objective To investigate the influence of microRNA （miRNA, miR）-31 on cell proliferation and migration of colorectal cancer. Methods The expression of miR-31 in colorectal cancer tissues and matched adjacent mucosa were measured by reverse transcriptase - polymerase chain reaction （ RT - PCR）. Then we detected the cell proliferation and migration of colorectal cancer cell lines transfected by miR-31 mimics. The expression of Ras p21 protein activator 1 （RASA1） coloreetal cancer cell lines transfected by miR-31 mimics were detected by Western blotting. Results MiR-31 in colorectal cancer cell lines and tissues were high-expression. Overexpression of miR-31 in colorectal cancer cell lines could promote cell proliferation and migration. Meanwhile, the expression of RASA1 in miR-31 mimics group was 0. 21± 0. 05, which was significantly higher than that in miR-31 inhibition group （ 2.46 ±0. 35 ）. Conclusion miR-31 may participate in the cell proliferation and migration of colorectal cancer cell lines, and the possible mechanism is targeting effect on RASA1.