肺腺癌相关微小RNA的筛选及其功能研究

Expression profiling and functional analysis of microRNA ~ted with pulmonary adenocarcinoma

目的筛选肺腺癌密切相关的微小RNA（miRNA，miR），为进一步研究其在肺腺癌发病机制中的作用奠定基础。方法收集肺腺癌及相应癌旁组织各4例，抽取总RNA，利用miRNA芯片技术，检测4例肺腺癌及其相应癌旁组织中miRNA的表达；采用实时定量反转录-聚合酶链反应（RT—PCR）方法验证miRNA芯片结果的可靠性；利用靶基因预测软件预测肺腺癌相关miRNA可能调控的靶基因，并初步分析其生物学功能。结果相对于癌旁组织，肺腺癌标本中有hsa—miR-155、hsa—miR-21、hsa—miR-550、hsa．miR-939、hsa—miR-30c-1、hsa-miR-146b-3p、hsa-miR-223和hsa—miR-194等8个miRNAs表达显著上调，差异倍数分别为82．72、38．51、16．06、9．69、3．27、3．14、2．65、2．18。hsa-miR-145、hsa-miR-339-5p、hsa-miR-203和hsa—miR-205等4个miRNAs表达显著下调，差异倍数分别为15．12、10．28、5．17、3．78。结论筛选得到的与肺腺癌关系密切的miRNA，可能在肺腺癌发生发展过程起重要的调控作用。

Objective To screen and identify the differential expressed microRNA （miRNA, miR） associated with pulmonary adenocarcinom （PA） to make further study on the function of miRNA in the pathogenesis of PA. Methods Total RNA was extracted from 4 PA samples and the corresponding normal adjacent tissues. MiRNA was hybridized on miRNA array. Real -time quantitative polymerase chain reaction （PCR） was applied to verify the reliability of miRNA array results. MiRNAs associated with PA were forecasted by target gene prediction software, including their preliminary biological functions. Results Compared with normal adjacent tissues, 8 miRNAs including hsa - miR - 155, hsa - miR - 21, hsa - miR - 550, hsa - miR - 939, hsa - miR - 30c - 1, hsa - miR - 146b - 3p, hsa - miR - 223 and hsa - miR - 194 were significantly increased, fold changes were 82. 72, 38.51, 16.06, 9. 69, 3.27, 3. 14, 2. 65 and 2. 18, respectively. 4 miRNAs including hsa - miR - 145, hsa - miR - 339 - 5p, hsa - miR - 203 and hsa - miR - 205 were significantly decreased, fold changes were 15.12, 10. 28, 5.17 and 3.78 respectively. Conclusion MiRNA differential expression profile of PA was obtained, which might play an important role in the development of PA.