六个海岛棉品系体细胞胚胎发生及再生体系的研究

The Study of Regeneration System from Different Sea Island Cotton(Gossypium barbadense L.)Cultivars

以海岛棉DJ-1(373)、天长2号(376)、5917(380)、阿长-599(381)、塔07-152(383)、S0717(408)6个品系的下胚轴为外植体,通过双因素随机区组设计的方法筛选出的两组最佳激素组合,诱导出愈伤组织,并成功获得再生植株。研究结果表明,0.1 mg/L 2,4-D+0.1 mg/L KT为海岛棉380、383和381愈伤组织最佳诱导条件,0.3 mg/L NAA+0.1 mg/L KT为海岛棉373、376和408愈伤组织最佳诱导条件;诱导出的愈伤组织接种于KNO3加倍的培养基可产生胚性愈伤组织;将其接种于含有0.1mg/L KT和0.05 mg/L IBA的培养基中可促进体细胞胚胎的发生和成熟,利用畸形苗的培育可缩短再生体系的周期,6个品种在6-8个月内均能获得再生植株。

The hypocotyls of 6 sea island cotton cultivars, i. e., DJ-1（373）, Tianchang 2（376）, 5917（380）, Achang-599（381）, Ta 07-152（383）, and S0717（408）were used as explant materials;calli were successfully induced with two optimized combinations of plant growth regulator（PGR）screened by the method of double factors randomized block, and the plants were regenerated successfully. The results showed that, the best PGR combination to induce callus for 380, 383, and 381 was 0.1 mg/L 2, 4-D ＋ 0.1 mg/L KT, while that for 373, 376, and 408 was 0.3 mg/L NAA ＋ 0.1 mg/L KT. The induced calli inoculated into the medium of double KNO3 generated embryogenic ones, and inoculating them into the medium of 0.1 mg/L KT and 0.05 mg/L IBA promoted the somatic embryogenesis and their maturing. The culture of malformed seedlings shortened the cycle of regeneration system, and the regenerated plants were all obtained from 6 cultivars in 6 to 8 months. A stable and efficient regeneration system without genotype limit was established.