马铃薯突变体赤霉素代谢关键酶基因差异表达分析

The Differential Expression of Genes for Key Enzymes in Gibberellin Metabolism in Potato Mutant

为揭示马铃薯株高突变与赤霉素代谢途径关键酶基因的关系,以马铃薯株高突变株系4P2-9为材料,利用实时荧光定量PCR方法中的相对定量RT-PCR建立双标准曲线,以Actin基因为内参基因,对高原4号及其株高突变材料4P2-9中的赤霉素代谢相关基因的表达情况进行了检测。结果显示,4P2-9的株高及节间数显著低于对照材料(P<0.05),节间长度显著高于对照(P<0.05),赤霉素代谢的关键酶基因KS、KO和GID1表达量显著下调,分别为对照材料的0.725倍、0.657倍和0.890倍;GA20ox1和GA2ox1基因的表达量表现为上调,分别为对照材料的1.557倍和1.425倍。结果表明,赤霉素代谢过程中关键酶基因表达量的改变是造成4P2-9材料株高变化的原因之一,而GA20ox1和GA2ox1基因的表达量的上调是促使株高降低的主要因素。

In order to reveal the correlation between the mutation of plant height and genes of key enzymes in gibberellin（GA）metabolic pathway, using the mutant strain 4P2-9 of potato as materials, we established double standard curve by relative quantitative RT-PCR, and tested the expression of GA metabolism-related genes in the ＂Plateau 4＂ and its mutant ＂4P2-9＂ using Actin gene as reference gene. The results showed that the plant height and the internode number of 4P2-9 significantly were lower than the control material（P〈0.05）;however, the internode length was significantly higher than the control（P〈0.05）, moreover the expressions of gene KS, KO and GID1 for key enzymes in GA metabolism were significantly down-regulated, and were 0.725, 0.657 and 0.890 times the control, respectively. The expressions of GA20ox1 and GA2ox1 genes were increased, and were 1.425 and 1.557 times the control, respectively. The results revealed that the expression changes of genes for key enzymes in the GA metabolism was one reason of the variation of 4P2-9 plant height, and the up-regulation of gene GA20ox1 and GA2ox1 expression was the main factor for the decrease of plant height.