热休克蛋白27在烟草烟雾提取物介导的牙龈成纤维细胞损伤中的表达变化

Expression of heat shock protein 27 in cigarette smoke extract-induced injury of human gingival fibroblasts

目的观察在不同质量分数烟草烟雾提取物（CSE）干预下，热休克蛋白（HSP）27在人牙龈成纤维细胞（HGFs）损伤过程中的表达。方法取原代培养并经过鉴定的3～8代HGFs，采用细胞划痕试验检测不同刺激质量分数（0、2．5％、5．0％、12．5％、25．0％、50．0％）的CSE对HGFs体迁移的影响，并采用Westernblot方法检测HSP27在HGFs中的表达。结果CSE质量分数越高，细胞的迁移能力越弱；HSP27在正常HGFs中呈弱阳性表达，在CSE刺激后的HGFs中呈强阳性表达，且随CSE质量分数的增高，HSP27的相对表达量有逐渐增高的趋势，与细胞迁移能力相反。结论HSP27在CSE介导的HGFs损伤中表达升高，在CSE介导的上皮损伤中有重要作用。

Objective This research aimed to observe the expression of heat shock protein 27 （HSP27） in human gingival fibroblasts （HGFs） cell injury induced by different concentrations of cigarette smoke extract （CSE）. Methods The third to eighth generations of cultured HGFs were treated with serially diluted CSE of different concentrations （0, 2.5%, 5.0%, 12.5%, 25.0%, 50.0%）. Wound-healing assay was performed to determine the migration of HGFs, and Western blot was used to deter- mine the expression of HSP27. Results The migration capability of HGFs weakened with the increase of CSE concentration. HSP27 expression was negative in normal HGFs but positive in CSE-intervened HGFs in a concentration-dependent manner. Conclusion HSP27 concentration increased in the CSE-induced injury of HGFs. This finding suggests that HSP27 plays an important role in CSE-induced epithelial injury.