转化生长因子-β1和白细胞介素-10单核苷酸多态性与复发性口腔溃疡易感性的研究

Relationship between transforming growth factor-β1 and interleukin-10 single nucleotide polymorphism and susceptibility of recurrent aphthous ulcer

目的研究转化生长因子-β1（TGF-β1）-509T／C位点与白细胞介素-10（IL-10）-1082A／G位点的单核苷酸多态性与复发性口腔溃疡（RAU）易感性的相关性。方法采用限制性片段长度多态性-聚合酶链式反应（RFLP-PCR）法和序列特异性引物．聚合酶链式反应（SSP-PCR）法对138例RAU患者和124例健康对照者进行TGF-β1-09位点与IL-10-1082位点的单核苷酸多态性的检测分析，用比值比（OR）和95％可信区间（95％CI）估计相对危险度。结果TGF-β1-09位点与IL-10-1082位点在基因型频率与等位基因频率的分布上，病例组与正常对照组之间均存在明显差异（P〈0．05）。TGF-β1-509位点基因型CT（OR=1231，95％CI=0．702-2．160）与TT（OR=2．482，95％CI=1．250-4．927）为高风险基因型，T等位基因为高风险等位基因（OR=1．465，95％CI=1．036～2．074）。IL-10-1082位点基因型AG（OR=1391，95％CI=0．808～2．396）与GG（OR=4．165，95％CI=1944～8．924）为高风险基因型，G等位基因为高风险等位基因（OR=2．134，95％CI=1．474～3．089）。结论TGFβ1-509位点与IL-10-1082位点是RAU患者的易感基因位点。TGF-β1-09位点携带T等位基因患RAU的风险性是携带C等位基因者的1．465倍。IL-10-1082位点携带G等位基因患RAU的风险胜是携带A等位基因者的2．134倍。

Objective To explore the possible relationship between recurrent aphthous ulcer （RAU） and single nucleotide polymorphism （SNP） of transforming growth factor-β1 （TGF-β1） -509T/C and intefleukin- 10 （IL- 10） - 1082A/G sites. Methods A total of 138 RAU patients were recruited for this study. The control group consisted of 124 subjects. TGF-β1-509T/C and IL-10-1082A/G sites were detected by restriction fragment length polymorphism-polymerase chain reaction （RFLP-PCR） and sequence specific primer-polymerase chain reaction （SSP-PCR）. Relative risk ratios were estimated by odds ratios （OR） and 95% confidence interval （95%CI）. Results Significant differences were found in the genotype frequencies or allele frequencies of TGF-β1-509T/C and IL-10-1082A/G sites between the RAU patients and controls （P〈0.05）. CT genotype （OR=1.231, 95%CI=0.702-2.160）, TT genotype （OR=2.482, 95%CI=1.250-4.927）, and T allele （OR=1.465, 95%CI=1.036- 2.074） at the TGF-131-509 site exhibited high risks. AG genotype （OR=1.391, 95%CI=0.808-2.396）, GG genotype （OR=4.165, 95%CI=1.944-8.924）, and G allele （OR=2.134, 95%CI=1.474-3.089） at the IL-10-1082A/G site also showed high risks. Conclusion TGF-131-509T/C and IL-10-1082A/G sites are associated with the risk of RAU. The TGF-β1 gene-509T alleleand IL-10 gene-1082G allele may serve as genetic determi- nants for RAU.