试验性豆状囊尾蚴病兔的组织病理学观察

Observation on histopathological changes of the rabbits infected experimentally with Cysticercus pisiformis

为观察家兔豆状囊尾蚴病不同时期的病理学变化,分别在人工感染家兔后第35天、第60天采取被感染家兔的心、肝、脾、肾、肺等脏器样本,经固定,制作石蜡切片,HE染色后观察。结果显示,感染后第35天家兔的肝出现空泡变性和颗粒变性;部分肺泡扩张;肾小管颗粒变性。感染后第60天家兔主要表现为慢性间质性肝炎、慢性肾炎、慢性肺泡气肿;心肌纤维萎缩、断裂;脾白髓增多等。特征性病理变化为肝出现六钩蚴移行所致的出血、坏死及形成大小不等的肉芽肿。上述观察结果显示了感染后不同时期家兔心、肝、脾、肾、肺的一般性病理变化及肝的特征性病理变化,为进一步探讨这些病理变化的发生机制奠定了基础。

To observe the changes of histopathological lesions in the rabbits infected experimentally with Cysticercus pisiformis in different period,the tissues including livers,hearts,lungs,kidneys and spleens were collected on 35 th and 60 th day post-infection,respectively.We analyzed the formalin-fixed and paraffin-embedded samples,which were then stained with haematoxylin and eosin.After 35 days of the infection,both vacuolar degeneration and granular degeneration were found in the livers from the C.pisiformis infected rabbits,as well as the cavity of alveolus expanded unregularly;granular degeneration in renal tubule.The results showed that the rabbits exhibited histopathological lesions on 60 th day post-infection including chronic nephritis,chronic interstitial hepatitis and chronic alveolar emphysema.Moreover,60 th day post-infection led to cardiac muscle fiber atrophy and breakage,accompanying with the increased areas of the splenic white pulp.Besides,the zones of hemorrhage and necrosis caused by the migration of oncospheres and granulation tumors were formed on the surface of livers.In summary,we demonstrate the different organic lesions of the rabbits infected with C.pisiformis.The results lays the foundations for the elucidation of the characteristic pathological changes and the general pathological changes during C.pisiformis infection.