辣椒粕分离蛋白酶解工艺条件的优化

Optimization on enzymatic hydrolysis processing from pepper meal protein isolate

从辣椒粕中提取粗蛋白,建立蛋白酶水解辣椒粕分离蛋白制备呈味肽的最佳条件。以水解度和肽得率为指标,对胰蛋白酶、木瓜蛋白酶、碱性蛋白酶、复合蛋白酶4种酶水解辣椒粕分离蛋白的效果进行比较研究。结果表明:复合蛋白酶的水解效果最佳。通过单因素试验选取复合蛋白酶作用的固液比、酶用量、酶解时间、酶解pH值及酶解温度作为试验因素,初步确立复合蛋白酶的水解条件。并通过正交试验优化最终确定复合蛋白酶水解辣椒粕分离蛋白的最佳工艺条件为固液比110(mV),酶用量2.0%,酶解时间4h,酶解pH值6.5,酶解温度50℃。通过实验验证,在该条件下,复合蛋白酶对辣椒粕分离蛋白具有较好的水解效果,其水解度为19.60%,肽得率为6.30%。

Extraction of crude protein from pepper meal, by the establishment of protease hydrolysis pepper meal protein isolate preparation in the best condition of flavor peptides was studied. Through judging the degree of hydrolysis and yield of peptide, the compound protease had the best hydrolyzation effect in digesting pepper meal protein isolate among the trypsin, papain, alkaline, compound protease. The results showed that compound protease had best effect. Selecting solid-liquid ratio （m ： V）, amount of enzyme, hydrolysis time, pH and hydrolysis temperature as experiment factors, the best technological condition of digesting pepper meal protein isolate was determined by method of single factor experiment and orthogonal experiment. The result showed that the best effect of Compound protease was in the condition of solid-liquid ratio（m ： V）1 ： 10, enzyme dosage 2.0%, pH 6.5, time 5 h and 50 ℃. On the conditions, compound protease has better hydrolyzation effect on digesting pepper meal protein isolate, after digesting the degree of hydrolysis is 19.60%, yield of peptide is 6.30%.