大鼠活体内Luciferase标记骨髓间充质干细胞生物发光示踪监测

In vivo monitoring of luciferase labeled bone marrow mesenchymal stem cells by bioluminescent in rats

目的应用生物发光技术监测同种异体骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSC)在健康大鼠体内的定植与动态分布情况。方法 Wistar大鼠BMSC经稳定表达增强绿色荧光蛋白(enhanced green fluorescent protein,e-GFP)和荧光素酶(luciferase)基因的慢病毒感染后,经尾静脉注射植入Wistar大鼠体内,通过活体成像连续监测其动态分布。结果活体成像结果显示,植入感染病毒的骨髓间充质干细胞(Luc-GFP-BMSC)1.5 h后在大鼠体内生物信号最强,主要聚集在肺,24 h后背部信号增强并居于主导,第3天胸部信号检测不到,14 d后,全身生物信号消失。结论同种异体大鼠BMSC经尾静脉植入后,首先聚集于肺部,弥散分布于背部,最终信号逐渐消失,提示经尾静脉注射植入可能是BMSC治疗肺部疾病的有效途径。

Objective To monitor the colonization and dynamic distribution of allogeneic bone marrow mesenchymal stem cells（BMSC） in healthy rats by using the technique of bioluminescent imaging. Methods BMSC from Wistar rat were infected by lentiviral which carry stable expression genes of luciferase and green fluorescence protein（Luc-GFP-BMSC）, then implanted into Wistar rats by tail vein injection. Dynamic distribution of Luc-GFP-BMSC in rats was continuously monitored by using in vivo imaging system. Results In vivo imaging results showed that biological signal was strongest at 1.5 hours after implantation of Luc-GFP-BMSC in rats, and mainly resided in the lung, 24 hours later, signal in the back gradually enhanced and became dominant, after 3 days, signal in the chest couldn＇t be detected, after 14 days, biological signal in the whole body disappeared. Conclusions After implantation through the tail vein, allogeneic BMSC in healthy Wistar rats initially reside in the lungs, and then egress to the back, finally the biological signal gradually disappeared, which suggests that tail vein injection of BMSC may be an effective approach for lung disease.