830nm激光穴位照射对力竭运动大鼠骨骼肌SOD与MDA含量的影响

Effect of Acupoint 830 nm Laser Irradiation on Skeletal Muscle SOD and MDA Contents in Exhaustive Exercise Rats

目的观察830 nm半导体激光穴位照射对力竭运动大鼠骨骼肌内SOD与MDA含量的影响。方法采用大鼠跑台下坡跑,进行力竭离心运动。将大鼠随机分为空白组(A组)、模型组(B组)、运动前半导体激光预照射组(C组)、运动后激光照射治疗组(D组)和运动前后激光照射联合组(E组),除空白组外,其余各组再分为24 h和48 h亚组,分别为B1组、B2组、C1组、C2组、D1组、D2组、E1组和E2组,每组8只。B组大鼠进行跑台一次性力竭运动;C组大鼠在力竭运动前激光预照射7 d,然后进行力竭运动;D组大鼠在运动后给予激光照射;E组大鼠在跑台运动前给予激光预照射7 d,运动后再给予激光照射治疗的联合照射。B1组、C1组、D1组和E1组在运动后24 h取材,B2组、C2组、D2组和E2组在运动后48 h取材。激光采用830 nm半导体激光发射器,穴位选取大鼠承山穴。观察各组治疗前后骨骼肌丙二醛(MDA)及超氧化物歧化酶(SOD)的含量。结果 B1组、D1组、B2组和C2组肌肉MDA含量与A组比较,差异均具有统计学意义(P<0.05)。D2和E2组肌肉MDA含量与B2组比较,差异均具有统计学意义(P<0.05)。B1组、C1组、D1组和B2组肌肉SOD含量与A组比较,差异均具有统计学意义(P<0.05)。C2组、D2组和E2组肌肉SOD含量与B2组比较,差异均具有统计学意义(P<0.05)。结论 830 nm激光穴位照射能够恢复失衡的氧化还原反应。

Objective To investigate the effect of acupoint 830 nm semiconductor laser irradiation on skeletal muscle SOD and MDA contents in exhaustive exercise rats. Methods Rats did exhaustive eccentric exercise by downhill treadmill running. Rats were randomized into a blank group（group A）, a model group（group B）, a pre-exercise semiconductor laser pre-irradiation group（group C）, a post-exercise laser irradiation group（group D） and a pre- and post-exercise laser irradiation combination group（group E）. Except the blank group, each of the other groups was again divided into 24 h and 48 h subgroups, and there were groups B1, B2, C1, C2, D1, D2, E1 and E2, 8 rats each. Group B rats did one exhaustive exercise by treadmill running. Group C rats received laser pre-irradiation for 7 days before they did exhaustive exercise. Group D rats received laser irradiation after the exercise. Group E rats received laser pre-irradiation for 7 days before treadmill running and combined laser irradiation after the exercise. Specimens were taken in groups B1, C1, D1 and E1 at 24 h after the exercise and in groups B2, C2, D2 and E2 at 48 h after the exercise. A 830 nm semiconductor laser transmitter was used for laser irradiation. Point Chengshan was selected in the rats. Skeletal muscle superoxide dismutase（SOD） and malondialdehyde（MDA） contents were measured in every group before and after treatment. Results There was a statistically significant difference in muscular MDA content between group B1, D1, B2 or C2 and group A（P〈0.05） and between group D2 or E2 and group B2（P〈0.05）. There was a statistically significant difference in muscular SOD content between group B1, C1, D1 or B2 and group A（P〈0.05） and between group C2, D2 or E2 and group B2（P〈0.05）. Conclusion Acupoint 830 nm semiconductor laser irradiation can restore unbalanced oxidation-reduction reactions.