长枝木霉菌株T05液态发酵产孢和菌丝生物量条件的筛选

Optimal Conditions for Trichoderma longibrachiatum Stain T05 for Producing Conidia and Chlamydospores

采用PDB培养基,对长枝木霉(Trichoderma longibrachiatum)菌株T05液态发酵产分生孢子、菌丝和厚垣孢子的理想条件进行了筛选,分别测试了培养温度、培养液初始pH、供氧量和初始接菌量的影响。结果表明:在150 r·min^(-1)振荡培养条件下,菌株T05产生分生孢子的适宜液态发酵条件为,培养温度30℃、培养液初始pH=12、供氧条件为250 m L的三角瓶装瓶量40 m L、250 m L的三角瓶装瓶量100 m L的情况下初始接菌量为0.5 m L,在这样的培养条件下培养5~7 d;产生菌丝和厚垣孢子的适宜液态发酵条件为,培养最适温度较低为20℃培养5 d;培养液初始pH值接近中性为6;供氧条件是初始装液量较多较有利,为250 m L的三角瓶装瓶量160 m L;初始接菌量是较少较有利,为250 m L的三角瓶装瓶量100 m L的情况下接菌量为0.05 m L。

We screened the optimal liquid fermentation conditions for Trichoderma longibrachiatum strain T05 to produce eonidia and hyphae biomass in PDB medium, four factors including culture temperature, initial pH value of culture solution, oxy- gen supply quantum, and tested initial inoculum volume for sporulation quantity. Under 150 r · min-1 oscillatory incuba- tion, the optimal conditions for T05 to produce conidia were the temperature of 30 ℃, the initial pH of 12, 40 mL PDB medium bottled in 250 mL conical flask, and the initial inoculum volume 0.5 mL in 100 mL PDB in 250 mL flask for 5-7 d culture, and the optimal conditions to produce mycelia and chlamydospores were the culture temperature of 20 ℃, the in- itial pH of 6, 160 mL PDB medium bottled in 250 mL conical flask, and the initial inoculum volume 0.05 mL in 100 mL PDB in 250 mL flask for 5 d.