NO/ADMA在布鲁菌侵染小鼠机体过程中的响应特征

Response Characteristics of Nitric Oxide/Asymmetry Dimethyl Arginine in Process of BrucellaInfection in Mice

本试验旨在研究布鲁菌侵染小鼠过程中一氧化氮(NO)的作用,以及NO和非对称性二甲基精氨酸(ADMA)在此过程的相互作用关系。以布鲁菌标准疫苗株M5侵染小鼠,首先用小鼠的血清进行虎红平板凝集(RBPT)和试管凝集试验(SAT),再用Griess试剂法和ELISA法测定对照组和侵染组不同组织和血清中的NO和ADMA含量,对小鼠肝脏和脾脏的各个侵染时间段进行CFU计数观察。结果显示,用布鲁菌M5侵染小鼠后,RBPT和SAT在14d时检测到有阳性反应。侵染组和空白对照组NO总含量上变化不大,但侵染组血清中的NO含量随时间出现下降,而肝脾中NO含量随时间出现上升,其他各组织NO含量波动不明显。而ADMA的总趋势与NO相反。CFU计数结果显示,小鼠肝脾内布鲁菌的数量在14d时一直处于增长状态,28d时布鲁菌的数量下降,表明布鲁菌的分裂繁殖和机体NO的含量存在一定的关系。

This experiment was aimed to study the role of nitric oxide （NO） in the process of Brucella in- fection in mice, as well as the interaction between NO and asymmetric dimethyl-arginine （ADMA） in this process. Brucella vaccine strain M5 was used to infect mice, firstly the red plate agglutination （RBPT） and tube agglutination test （SAT） were carried out using the sera of mice. The contents of NO and ADMA in different tissues and sera between control group and infection group were detected by Griess reagent method and ELISA method. At the same time, the CFU counts of mice＇s liver and spleen were observed in each infection period. The results showed that positive reaction were detected in 14 d by RBPT and SAT experiments after using Brucella M5 infection mice. Total NO contents changed little between infection groups and blank control groups. But the contents of NO in the infection group decreased with time, the contents of NO in liver and spleen increased with time, the concents of NO in other tissues were not fluc- tuated obviously. While the trend of ADMA contents was contrary to the NO contents. Results of CFU counts showed that the number of Brucella in the liver and spleen rose before 14d, and fell at 28 d. It sug- gested that there is a certain relationship between the proliferation of Brucella and the NO contents.