甾类激素对原代培养附睾头部上皮细胞中Bin1b表达的影响

Influence of steroid hormone on expression of Bin1b in primary cultured epididymal epithelial cells

目的探讨甾类激素对原代培养的大鼠附睾头部上皮细胞中Bin1b表达的影响。方法不同浓度(10^(-9),10^(-8),10^(-7) mol/L)的二氢睾酮(dihydrotestosterone,DHT)、(10^(-9),10^(-8) mol/L)雌二醇(estradiol,E2)及(10^(-8),10^(-7) mol/L)地塞米松(dexamethasone,Dex)处理原代培养的附睾头部上皮细胞0~5d,分别采用1.2%琼脂糖凝胶电泳和PCR的方法检测Bin1b mRNA的表达。结果原代培养的附睾上皮细胞中Bin1b mRNA的表达呈时间依赖性减少,培养第2天Bin1b mRNA水平下调约40%,第3天下调约70%,5d时附睾上皮细胞中几乎检测不到Bin1b mRNA的表达。不同浓度的DHT对Bin1b mRNA表达的下降有不同程度的减缓,10-9 mol/L的DHT使Bin1b mRNA表达较对照细胞增加约37%(P<0.05),10^(-8) mol/L及10^(-7) mol/L DHT处理后Bin1b mRNA表达进一步的增加。不同浓度的E_2和Dex对Bin1b mRNA表达的下降无明显调节作用。结论雄激素能上调原代培养的附睾头部上皮细胞中Bin1b的表达,而雌激素和糖皮质激素没有明显的调节作用。

Objective To study the expression of Binlb in primary cultured epididymal epithelial cells treated with different steroid hormones at various concentrations. Methods Expression of Binlb mRNA was examined by agarose gel electrophoresis and PCR in primary cultured epididymal epithelial cells, which were treated with dihydrotestosterone （DHT, 10-9,10-8 and 10 -7 mol/L）, estradiol （E2, 10-9 and 10 -8 mol/L）） or dexamethasone （Dex, 10-8 and 10-7 mol/L） at different concentrations. Results The expression of Binlb mRNA in primary cultured epididymal epithelial cells was decreased in a time- dependent manner. The mRNA level of Binlb was decreased by 40% at day 2 and 70% at day 3; and it was hardly detectable at day 5. DHT up-regulated the expression of Binlb mRNA in a dosage-dependent manner compared with corresponding vehicle control. 10 9mol/L DHT up-regulated Binlb mRNA level by 37~/oo compared with corresponding vehicle control（P〈0. 05）, and it was further up-regulated after the treatment with 10-8mol/L and 10- 7 mol/L DHT. E2 and Dex at various concentrations showed no significant effects on the expression of Binlb mRNA in primary cultured epididymal epithelial cells. Conclusion Dihydrotestosterone can up-regulate the expression of Binlb mRNA in primary cultured epididymal epithelial cells, while estradio and dexamethasone can not.