改良Frey氏液体培养基质控菌株菌种代次与生长曲线研究

Study on the Generation and Growth Curve of Modified Frey Liquid Culture Medium Quality Control Strains

为探究改良Frey氏液体培养基质控菌CVCC2960滑液支原体（以下简称MS）生长规律，本试验首次同时采用CFU计数、CCU测定和pH值测定的方法，比较不同接种浓度、接种体积和不同代次MS生长情况。结果显示：5％与10％接种量的CCU测定结果峰值差异不明显，但5％接种的CFU计数峰值更高，pH下降更慢；1～5代菌在生长曲线各阶段的测定结果均无明显差别；3种不同接种方式的测定结果显示，用0．5mL菌液接种9．5mL培养基的方式更利于MS生长。本试验为改良Frey氏液体培养基的质控标准化提供依据。

To explore the growth law of a quality control strain for modified Frey liquid medium, CVCC2960 Mycoplasma Synoviae （MS）, the colony counting method, CCU assay and pH determination were combined to compare MS growth in different inoculation concentrations, inoculation volumes and generations. The results indicated that there was no significant difference in growing titer peak, while 5% strains had a higher living cell concentration peak and a slower decrease pH value. The generation and growth value of 1 - 5 generations didn＇ t have significant difference. That mix 0.5 mL bacteria suspension with 9.5 mL medium was proved to be more suitable than other two for MS. This study provided a basis evidence of standardization for modified Frey liquid medium.