一株新型鸭肝炎病毒的分离与鉴定

Isolation and Identification of a Duck Hepatitis Virus Strain

从辽宁省某鸭场疑似鸭病毒性肝炎病料中分离到一株病毒,经PCR检测初步鉴定其为鸭肝炎病毒,命名为YK株,应用易感鸭胚和雏鸭进行传代及病毒含量测定,E_1~E_5代鸭胚适应毒病毒含量在10^(6.5)~10^(6.9)ELD_(50)/0.2 m L之间,E2代鸭肝组织毒病毒含量为10^(6.3)LD_(50)/0.1 m L。动物试验结果表明,YK株鸭胚适应毒E_2代对7日龄雏鸭的致死率高达100%。序列测定分析表明,YK株与DHV-A的VP1基因同源性在71.4%~72.3%之间,与DHV-B的VP1基因同源性在71.9%~72.3%之间,与DHV-C的VP1基因同源性在94.3%~98.8%之间。试验表明,YK株与韩国新型鸭肝炎病毒在同一分支上,属于基因C型DHV。

A virus strain was isolated from suspected duck viral disease from dead duck in liaoning Province, and identified by reverse - transcriptase polymerase chain reaction. It is a duck hepatitis virus that named YK strain. The isolates were passed from E1 to Es, the titer determination in duck embryos were from 10^6.5 to 10^6.9 ELD50/0.2 mL; the titer determination in duckling hepatitis tissue titer was 10^6.3/0. lmL at E2. Animal regression experimental results showed that The YK strain in duck embryos to 7 - day - old duckling mortality was 100%. Sequence comparison with DHV - A serotype showed that the identity was 71. 4% - 72. 3% , 71.9% -72.3% with DHV -B serotype and 94.3% -98.8% with DHV -C serotype. It proves that the YK strain and new serotype strain in Korea belongs to DHV -C serotype.