水杨酸处理木槿种子后幼苗的抗逆生理效应研究

Physiological Characteristics of Anti-adversity of Hibiscus syriacus Seedlings from Salicylic Acid Treated Seeds

为在木槿育苗中合理应用水杨酸,以木槿当年生种子为试验材料,用0、30、60、120mg·L^(-1)和240 mg·L^(-1)5个水杨酸浓度,3、7、20℃3个温度进行处理,研究了幼苗叶片中3种生理活性物质的含量。结果表明:120mg·L^(-1)浓度处理可溶性蛋白在3、7、20℃时分别比对照提高了90.01%、86.50%、22.33%,3℃和7℃下差异显著;可溶性糖含量3℃、7℃下比对照提高22.61%、18.4%,3℃下差异显著;3个温度下游离脯氨酸含量分别升高73.24%、112.56%、40.10%,差异显著;MDA含量分别降低44.02%、50.28%、39.18%,差异显著;电导率分别比对照降低47.51%、32.21%、21.78%,其中3℃下与对照差异显著。综合分析认为,水杨酸浓度为120mg·L^(-1)时对提高木槿叶片内渗透物质含量和降低MDA含量以及电导率效果最佳。

In order to reasonably apply salicylic acid （SA） in breeding Hibiscus syriacus seedlings,annually produced seeds were treated by 5 levels of SA：0 （control） ,30,60,120,and 240 mg· L^-1 ,and cultured at 3, 7,and 20℃. At seedling stage,3 physiologically active components in the leaves were measured. The results showed that the contents of soluble protein in 120 mg · g^-1 treatment cultured at 3,7, and 20℃ were 90.01~,86.50% ,and 22.33% higher than that of the control; soluble sugar contents at 3 and 7 ℃ were 22.61% and 18.4% higher than that of the control; free proline contents at 3,7 and 20 ℃ were 73.24%, 112.56% ,and 40. 10% higher than that of the control; MDA contents at three temperatures were 44. 02% ,50.28% ,and 39.18% lower than that of the control; electric conductivities at 3 temperatures were 47.51%,32.21%,and 21.78% lower than that of the control. It was concluded that 120 mg. L lwasthe optimal concentration to improve the contents of the material within the hibiscus leaf penetration and decrease MDA content and conductivity.