摘要
以柴达木盆地梭梭同化枝为材料,扩增其P5CS基因并进行序列测定。结果表明:所得柴达木盆地梭梭P5CS基因的碱基数量为442个,编码146个氨基酸。另外,同源性比对分析结果显示梭梭与甜菜、可可等植物的P5CS基因具有较高的同源性。研究结果将为下一步梭梭P5CS基因的结构与功能研究奠定基础。
The fragment of P5CS gene of assimilating branches of Haloxylon ammodendron in Qaidam Basin was amplified and sequenced by the specific primers,then the nucleotide sequence and amino acid se- quence of P5CS gene was identified and summarized. The sequence analysis indicated that the total length of P5CS geng was 442 bp to encode 146AA. Homology analysis showed that there had high homology among Haloxylon ammodendron P SCS gene and other plants. This results would lay foundation for the further study of the structure of PSCS gene and it＇s function in H. ammodendron.
出处
《西北林学院学报》
CSCD
北大核心
2016年第1期88-91,共4页
Journal of Northwest Forestry University
基金
青海省科技厅自然科学基金项目(2012-Z-938Q)
关键词
梭梭
P5CS基因
扩增
序列分析
HaloxyZon ammodendron
P5CS gene
amplification
sequence analysis
