下调CD133表达对肝癌细胞恶性生物学行为的影响

Impact of CDl33 expression down-regulation on malignant biological behaviors of liver cancer cells

目的:探讨下调CDl33基因的表达对肝癌细胞生物学行为的影响。方法:将合成的CDl33小干扰核糖核酸分子(si RNA)转染至肝癌SMMC7721细胞并检测转染效率;分别以无转染与转染随机si RNA序列的SMMC7721细胞为空白对照和阴性对照,观察CDl33 si RNA转染后CDl33基因沉默效果,以及SMMC7721细胞主要生物学行为的变化。结果:转染24 h后,转染效率可达到(80.8±9.1)%;与空白对照组比较,CDl33 si RNA转染后的SMMC7721细胞CD133 m RNA及蛋白表达量分别降至空白对照组的10%与35%、细胞增殖活性明显降低、细胞凋亡率明显增加(41.3%vs.25.3%)并出现明显的S期阻滞,集落形成能力明显降低(均P<0.05)。阴性对照组与空白对照组各指标无统计学差异(均P>0.05)。结论:CDl33在肝癌细胞中可能起了癌基因作用,下调其表达能抑制肝癌的恶性生物学行为。

Objective： To investigate the influence of downregulating CD133 gene expression on biological behaviors of human liver cancer cells. Methods： Liver cancer SMMC7721 cells were transfected with the synthesized CD133 siRNA and then transfection efficiency was determined. Using SMMC7721 cells without transfection or transfected with scrambled siRNA sequence as blank control and negative control respectively, the effect of CD 133 gene silencing, and changes in the main biological behaviors in SMMC7721 cells after CD133 siRNA transfection were observed. Results： The transfection efficiency reached （80.8±9.1） % at 24 h after transfection. Compared with blank control group, in SMMC7721 cells after CD133 siRNA transfection, the expression level of CD133 mRNA and protein was reduced to 10% and 35% of the level in blank control group respectivel）5 the proliferative activity was significantly decreased, apoptosis rate was significantly increased with a marked S-phase arrest, and colony-forming ability was significantly decreased （all P〈0.05）. All indexes did not show any significant difference between negative control group and blank control group （all P〉0.05）. Conclusion： CD133 gene may play an oncogene role in liver cancer cells and down-regulating its expression can suppress the malignant activity of liver cancer.