JAK2/STAT3信号通路在肝细胞性肝癌中表达及意义

Expression of JAK2/STAT3 signaling pathway in human hepatocellular carcinoma and its significance

目的:探讨JAK2/STAT3信号通路在人肝细胞性肝癌(HCC)组织中的表达以及其意义。方法:用免疫组化法和Western blot法检测75例HCC组织及其相应的癌旁组织JAK2与STAT3蛋白的表达,分析两者与HCC患者病理特征及预后的关系。结果:JAK2与STAT3蛋白在HCC组织中的阳性表达率及表达量均高于相应的癌旁组织(62.7%vs.5.3%,69.3%vs.9.3%;均P<0.05);JAK2与STAT3蛋白在HCC组织中的表达呈明显正相关(r=0.383,P<0.01)。JAK2和STAT3蛋白的表达与肝硬化、门静脉癌栓、肿瘤分化程度、临床分期明显有关(均P<0.05)。生存分析显示,JAK2与STAT3蛋白高表达患者的生存率及生存期均明显低于各自的低表达患者(χ2=13.591;χ2=6.842,均P<0.05);Cox比例风险回归模型分析表明,JAK2与STAT3蛋白以及门静脉癌栓、肿瘤分化程度、临床分期均为影响HCC预后的独立危险因素(均P<0.05)。结论:JAK2/STAT3信号通路在HCC组织中活性增高,且其活性高低与HCC患者预后密切相关。

Objective： To investigate the expression of JAK2/STAT3 （HCC） tissue and its significance. Methods： The protein expressions of JAK2 and STAT3 in signaling pathway in human hepatocellular carcinoma specimens of HCC tissues and matched adjacent noncancer tissues from 75 patients were detected by immunohistochemical staining and Western blot analysis. The relations of their expressions with the clinicopathologic features and prognosis of the patients were analyzed. Results： Both positive expression rate （62.7% vs. 5.3%; 69.3% vs. 9.3%） and expression level of either JAK2 or STAT3 protein in HCC tissue were significantly higher than those in the adjacent non-cancer tissue （all P〈0.05）; there was significant correlation between the expressions of JAK2 and STAT3 protein in HCC tissue （r=-0.383,P〈0.01）. Both expressions of JAK2 and STAT3 were significantly related to cirrhosis, portal vein tumor thrombus, degree of tumor differentiation, and clinical stage （all P〈0.0S）. Survival analysis showed that both survival rate and survival time in patients with high JAK2 or STAT3 expression were significantly decreased compared with corresponding low expression cases （~=13.591; ~=6.842, both P〈0.05）. Cox proportional hazard regression model analysis indicated that the expression of JAK2 and STAT3 protein along with portal vein tumor thrombus, degree of tumor differentiation, clinical stage were independent risk factors for HCC （all P〈0.05）. Conclusion： The activity of the JAK2/STAT3 signaling pathway is increased in HCC tissue and the degree of its activation is closely related to the prognosis of I--ICC patients