GA_3对乌饭树种子发芽特性的影响

Effect of GA_3 on Seed Germination Characteristic of Wufanshu(Vaccinium bracteatum)

乌饭树作为蓝莓嫁接的优良砧木,自然条件下种子发芽率很低。为探究赤霉素（GA3）对乌饭树种子发芽的影响,本研究设置9种不同浓度GA3和蒸馏水（对照组）浸种处理24 h,25℃恒温培养24天。结果表明：第12-18天为种子发芽高峰期,第16-24天,各GA3处理组的发芽率均高于对照组,第24天时800 mg/L GA3处理组的发芽率最高,达81.33%,比对照组提高了33个百分点,100-600 mg/L GA3处理的种子发芽率次之,为73.33%-76.17%,而50、1000 mg/L GA3处理组的发芽率稍低,分别为63.83%和67.33%。另外,100-800 mg/L GA3处理的发芽势和发芽指数均高于对照组,以800 mg/L GA3处理组最明显。可见,800 mg/L GA3浸种处理24 h对促进乌饭树种子发芽的效果最显著,此方法可在乌饭树种子繁殖实践中借鉴应用。

Wufanshu（Vaccinium bracteatum Thunb.） is an excellent rootstock for blueberry（Vaccinium spp.）.Under natural condition, Wufanshu seeds have a very low germination rate. To investigate the effect ofgibberellic acid（GA3） on seed germination, Wufanshu seeds were soaked in solutions containing nine differentconcentrations of GA3 and distilled water（control） for 24 hours, and then incubated at 25℃ for 24 days. Theresearch showed that the peak of seed germination was from the 12 thto the 18 thday. More seeds germinated inthe GA3-treated groups than in the control group from the 16 thto the 24 thday. On the 24 thday, the highestproportion of germinated seeds（81.33%） was in the 800 mg/L GA3 treatment group（33% higher than that in thecontrol group）. The proportion of germinated seeds in the 100-600 mg/L GA3 treatment groups ranged from73.33% to 76.17%, but was lower in the 50 and 1000 mg/L GA3 treatment groups（63.83% and 67.33%,respectively）. The germination potential and indices of the 100-800 mg/L GA3 treatment groups, especially the800 mg/L GA3 treatment group, were higher than those of the control group. These results showed that an800 mg/L GA3 soaking treatment for 24 hours strongly promoted Wufanshu seed germination. This method may serve as a reference in the seed propagation of Wufanshu.