PI3K/AKT及其相关因子在结肠癌中的表达

The expressions of PI3K/AKT and its related factors in colon cancer

目的探讨PI3K/AKT信号通路中相关细胞因子PI3Kp110α、p-AKTser473、p-m TORser2448、Cyclin D1在结肠癌组织中的表达及其与临床各病理参数之间的关系。方法采用免疫组织化学法检测65例结肠癌组织(结肠癌组)和15例结肠腺瘤组织(结肠腺瘤组)的PI3Kp110α、p-AKTser473、p-m TORser2448、Cyclin D1的表达,分析各指标在结肠癌组的表达与临床各病理参数之间的关系。结果PI3Kp110α、p-AKTser473、p-m TORser2448、Cyclin D1在结肠癌组中的阳性表达率均显著高于结肠腺瘤组,差异均有统计学意义(P<0.05)。PI3Kp110α、p-AKTser473、p-m TORser2448在结肠癌组的表达与性别、年龄、分化程度、是否淋巴结转移及TNM分期之间差异无统计学意义(P>0.05)。Cyclin D1在不同分化程度及不同TNM分期的结肠癌组织中的表达有统计学差异(P<0.05),在不同性别、年龄及是否有淋巴结转移之间无明显差异(P>0.05)。PI3Kp110α、p-AKTser473、p-m TORser2448、Cyclin D1在结肠腺瘤组的阳性表达与性别、年龄、分化程度、是否淋巴结转移及TNM分期之间无统计学差异(P>0.05)。结论 PI3Kp110α、p-AKTser473、p-m TORser2448、Cyclin D1的表达与结肠癌的发生发展密切相关,对结肠癌的预后评估及新靶点的研发具有重要意义。

Objective To investigate the expressions of PI3 K/AKT signaling pathw ay and its related factors（ PI3Kp110α,p-AKTser473,p-m TORser2448,Cyclin D1） in colon cancer and their association with other clinical pathological parameters. Methods The expressions of PI3Kp110α,p-AKTser473,p-m TORser2448 and Cyclin D1 were detected with immunohistochemical method in 65 cases of colon cancer（ colon cancer group）,and 15 cases of colonic adenoma（ colonic adenoma group）. The association between the expressions and clinical pathological parameters were analyzed. Results The positive expressions of PI3Kp110α,p-AKTser473,p-m TORser2448 and Cyclin D1 were significantly higher in colon cancer than in colonic adenoma（ all 〈P 0. 05）. In the colon cancer group,there were no differences in the expressions of PI3Kp110α,p-AKTser473 and p-m TORser2448 between different genders,ages,different degrees of differentiation,lymph node metastasis and TNMstaging（ all〉 P 0. 05）; while there were differences in the expression of Cyclin D1 between varying degrees of differentiation and TNMstaging（ all 〈P 0. 05）,but no statistical differences between different genders,ages and different degrees of lymph node metastasis（ all P 〈0. 05）. In thecolonic adenoma,there were no differences in the expressions of PI3Kp110α,p-AKTser473、p-m TORser2448 and Cyclin D1 between different genders,ages,degrees of differentiation,lymph node metastasis and TNMstaging（ all P〈 0. 05）. Conclusion The expressions of PI3Kp110α,p-AKTser473,p-m TORser2448 and Cyclin D1 are closely related with the occurrence and development of colon cancer,playing a significant role in the prognosis of colon cancer and new research targets.