石墨烯-壳聚糖复合材料修饰电极检测己烯雌酚

Electrochemical Immunosensor for Diethylstilbestrol Determination Based on Graphene-Chitosan Composite Modified Glassy Carbon Electrode

制备了石墨烯一壳聚糖复合材料修饰电极，采用循环伏安法研究复合材料对电极导电性能的影响，并利用己烯雌酚抗体与己烯雌酚之间的特异性应，以K2Fe（CN）6为探针，采用差分脉冲伏安法进行食品中己烯雌酚残留量的检测研究。在优化条件下，P（己烯雌酚）在1～2500Ixg／L内与电流差值呈线性关系，线性回归方程AI=25．0368＋0．00342p（R=0．99124），检出限为0．1Ixg／L。对猪肉、牛肉、鸭肉及奶粉样品进行加标检测，回收率93．2％～106．8％。

A graphene-chitosan modified glassy carbon electrode was prepared. Electrical conductivity of the modified glassy carbon electrode （GCE） was investigated by cyclic voltammetry （CV）. Competitive- electrochemical immunoassay was performed to detect diethylstilbestrol by differential pulse voltammetry （DPV）. Under the optimized conditions, the A-value of oxidation peak current was proportional to diethylstilbestrol concentration in the range between 1 and 2 500 μg/L. The linear equation was AI = 25. 036 8 ＋0. 003 42p （R =0. 991 24） , and the detection limit was 0. 1 μg/L. A good recovery of dieth- ylstilbestrol in the range of 93.2% -106.8% was obtained in pork, beef, duck, and milk powder.