牛卵母细胞体外成熟减数分裂进程及核型变化的研究

Research on the Bovine Oocyte Meiosis in-vitro Maturation and Karyotype Changes

为了更加深入了解牛卵母细胞体外成熟减数分裂及其伴随该进程中核型的变化,实验采用常规的牛卵母细胞体外成熟方法及核型染色方法对成熟过程中减数分裂恢复情况及其对应的核型进行了研究。结果表明:牛卵母细胞体外成熟8 h减数分裂抑制恢复率达45.26%,成熟12 h时绝大部分卵母细胞(81.63%)已经恢复了减数分裂抑制状态,正常成熟24 h后GVBD率达91.67%。说明在对牛卵母细胞进行体外成熟过程中卵母细胞可以按照正常的减数分裂进程发育至受精前阶段,经染色后可以较为清楚识别减数分裂各时期细胞核核型形态及其变化规律,为研究牛卵母细胞体外成熟减数分裂抑制恢复的分子机制探讨及体外成熟效率的提高提供了参考价值。

To further understand the bovine oocyte meiosis in-vitro maturation and karyotype changes along with the process, this paper studies the recovery status of meiosis in the process of maturation and the corresponding karyotype by the regular method of bovine oocyte in-vitro maturation and karyotype analysis. It is showed that the recovery rate of meiosis reaches 45.26% when bovine oocyte matures in vitro for 8 hours, while the rate reaches to 81.63%and 91.67%for 12 hours and 24 hours respectively, which demonstates that oocytes can grow and reach to the status prior to fertilization with normal meiosis process during the period of in-vitro maturation of bovine oocytes, and that the form of cellula karyotype and its rules in all period of meiosis can be identified clearly when the oocytes are dyed, which provides reference for the study of molecular mechanisms of bovine oocyte meiosis maturation and the improvement of efficiency of in-vitro maturation.