摘要
为探讨鸡传染性法氏囊病病毒(IBDV)BJ902株感染复数(Multiplicity of infection,MOI)、接种时间和维持液三种因素对在悬浮培养DF-1细胞上的病毒滴度影响,分别以不同MOI的IBDV BJQ902株感染悬浮培养24 h的DF-1细胞,以0.005 MOI的IBDV BJQ902株感染悬浮培养不同时间的DF-1细胞,然后以含2%胎牛血清(FBS)的高糖DMEM(HG-DMEM)作为维持液进行培养,观察感染后不同时间的细胞病变、收获感染不同时间的病毒液。以0.005 MOI感染悬浮培养24 h的DF-1细胞,然后使用含2%FBS的不同维持液(MEM、HG-DMEM、LG-DMEM)进行病毒增殖,收获不同时间的病毒液,测定病毒液的TCID_(50)。结果显示,以0.005 MOI的IBDV BJQ902种毒接种悬浮培养24 h的DF-1细胞,维持液为2%FBS的HG-DMEM进行病毒增殖,病毒接种后24~36 h收获的病毒毒价可达10^(8.3) TCID_(50)/0.1 m L以上。表明IBDV BJQ902株可以在微载体悬浮培养的DF-1细胞上高密度增殖。
The purpose of this study was to investigate the effects of multiplicity of infection （MOI） of infectious bursal disease virus （IBDV）BJQ902 strain, virus inoculation time and virus maintenance medium on virus titer inoculated in suspension culture of DF-1 cell. Suspension culture of DF-1 cells by 24 h was inoculated with different MOI of IBDV BJQ902 strain, cytopathic effect （CPE）was observed and virus titer was tested after inoculation every 12 hours. With 0.005 MOI of IBDV BJQ902 strain was inoculated in suspension culture of DF-1 cells which was cultured in different time, CPE was observed and virus titer was tested after inoculation every 12 hours. This study was also conducted when suspension culture of DF-1 ceils by 24 h was inoculated with 0.005 MOI of IBDV BJQ902 strain and then cultured with different maintenance medium （MEM, HG-DMEM,LG-DMEM）. The results showed that the virus titer could reach 10^8.3 TCID50/0.1 mL when suspension culture of DF-1 cells by 24 h inoculated with 0.005 MOI of IBDV BJQ902 strain and maintained with HG-DMEM containing 2% FBS. IBDV BJQ902 strain could be propagated with high efficiency in a suspension culture of DF-1 cell system.
出处
《中国家禽》
北大核心
2016年第1期16-20,共5页
China Poultry
基金
北京市农林科学院青年基金(QNJJ201409)
