副猪嗜血杆菌中毒素蛋白基因hipA的生物信息学分析

Bioinformatic Analysis of Toxin Protein Gene hipA from Haemophilus parasuis

为预测毒素蛋白HipA的二级结构和B细胞抗原表位,首先从GenBank中查找副猪嗜血杆菌毒素蛋白基因hip A的序列,应用生物信息学方法,对副猪嗜血杆菌毒素蛋白HipA的理化性质、二级结构和B细胞抗原表位进行预测。结果显示,HipA蛋白的理论等电点为7.23,前50个氨基酸为信号肽,二级结构的预测结果显示该酶主要以α螺旋、无规则卷曲和延伸链为主,只有少数的β转角;推测该蛋白有17个B细胞优势抗原表位区域。该研究为进一步研究分析副猪嗜血杆菌毒素蛋白HipA的功能奠定理论基础。

The aim of this study was to predict secondary structure and B-cell epitopes of toxin protein Hip A of Haemophilus parasuis. The hip A gene sequence was searched from Gen Bank. By using bioinformatics methods,the physicochemical property, the secondary structure and B-cell epitope of Hip A protein were predicted. Theresults showed that the IP of Hip A was 7.23 and the first 50 amino acids was signal peptide. Using the SOPMAmethod, prediction of the secondary structure of the Hip A showed that α-helix, random coils and extendedstrand were the main structural types of the flexible region in secondary structure, and there was a smallamount of β- turn. The Hip A protein was supposed to contain 17 potential antigen epitopes. These resultsprovided a theoretical basis for a further study of the toxin protein（Hip A） from Haemophilus parasuis.