咖啡酸苯乙酯对糖尿病大鼠肾保护作用

Protective effect of caffeic acid phenethyl ester on kidney of STZ-induced diabetic rats

目的探讨咖啡酸苯乙酯(CAPE)对糖尿病大鼠肾功能的保护作用及机制。方法将50只大鼠分为5组,对照组、模型组及CAPE低、中、高(12、24、48mg/kg)剂量组,一次性腹腔注射链脲佐菌素(STZ)65mg/kg制备糖尿病大鼠模型,第8周末测尿清蛋白(Alb)、视黄醇结合蛋白(RBP)和肌酐,腹主动脉取血,测定空腹血糖(GLU)和糖化血红蛋白(HbA1c)。取出肾脏,一部分肾组织用4℃生理盐水冲洗后,称质量研磨制成匀浆,再离心,取上清液测定丙二醛(MDA)水平、超氧化物歧化酶(SOD)和谷胱甘肽氧化物酶(GSH)的活性、一氧化氮(NO)水平、一氧化氮合酶(NOS)和Na-K-ATP酶活性。另一部分肾组织光镜下检测组织形态学变化。结果与模型组比较,CAPE高剂量组Alb和RBP明显降低(P<0.05);肾组织中NO、NOS、GSH和SOD活性明显上升,MDA水平明显下降,Na-K-ATP酶活性升高(P<0.05),肾组织病理损伤减轻。结论 CAPE能降低糖尿病大鼠肾脏损伤具有保护作用,起保护作用可能与提高肾脏抗氧化应激损伤有关。

Objective To investigate the effect of caffeic acid phenethyl ester（CAPE）on the kidney function of STZ-induced diabetic rats and its mechanism.Methods A total of 50 rats were divided into 5groups：conrol group,model group,CAPE low,middle and high doses（12,24,48mg/kg）groups.The diabetic rat model was established by a single injection of streptozotocin（STZ）65mg/kg.At the end of 8weeks,urine Alb,retinol binding protein（RBP）and creatinine were detected and blood glucose and HbA1 c were detected by collecting blood from abdominal aorta.The renal tissue was taken.The partial renal tissues were flushed by 4 ℃normal saline,weighed,grinded into homogenate and centrifuged and the supernate was taken for measuring the levels of malondialdehyde（MDA）,superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px）,NO,NOS and Na-K-ATP.The histomorphological changes in another part of renal tissues were observed under optical microscopy.Results Compared with the model group,the Alb and RBP levels in the CAPE 48mg·kg-1group were significantly decreased（P〈0.05）,NO,NOS,GSH and SOD levels in renal tissues were significantly elevated,while the MDA level was significantly decreased,the Na-K-ATPase activity was increased（P〈0.05）,the renal histopathological injury was alleviated.Conclusion CAPE can reduce the renal injury in STZ-induced diabetic rats and has the protective effect,which may be related with increasing the renal function for anti-oxidative stress injury.