外周血白细胞相对端粒长度与脑卒中的相关研究

Relative Leukocyte Telomere Length and Stroke in Chinese

目的探讨脑卒中患者外周血白细胞相对端粒长度的变化。方法入选初发脑卒中患者543例和健康对照组616例,提取外周静脉血白细胞基因组DNA,通过实时荧光定量聚合酶链反应法(real time-polymerase chain reaction,RT-PCR)检测相对端粒长度。通过Logistic回归模型分析脑卒中与端粒长度的关系。结果调整年龄、性别、血压、血糖、血脂等多种变量后,最低分位与最高分位端粒长度(三分位)相比,脑卒中的危险因素比值比(multivariate odds ratio,OR)以及95%可信区间(confidence interval,CI)为[1.05(0.72-1.51),p=0.4]。动脉粥样硬化亚型组的危险因素比值比(multivariate odds ratio,OR)以及95%可信区间(confidence interval,CI)为[2.33(1.42-3.83),p=0.003]。结论与对照组比较,动脉粥样硬化性脑梗死患者外周血白细胞相对端粒长度显著缩短。

Objective To investigate the association of relative telomere length（RTL） and stroke. Methods A total of543 stroke patients and 616 control subjects were recruited.There lativetelomere length ingeno micDNA extracted from circulating kocyte was measured by realtime quant it at ivepolymerase chain reaction. The telomeres was divided into tertile,and logistic regression analysis was used to determine the risk of stroke and relative telomere length. Results The multivar iate odds ratio [OR]（95% confidence inter val[CI]） was [1.05（0.72-1.51）, p= 0.4] for st roke and [2.33（1.42- 3.83）, p= 0.003] for atheroth rombotic stroke. Conclusion There was a significant association between shorten telomere length of leukocyte and atheroth rombotic stroke.