摘要
为鉴定新引进柱花草种质的遗传背景和亲缘关系，提高柱花草种质的利用效率，利用23个SSR标记对新引进的31份柱花草种质和4个柱花草栽培品种进行了遗传多样性分析。结果表明：23个SSR标记在35份柱花草种质问均具有多态性，共检测到等位基因134个，每个标记可检测到3～11个等位基因，平均为5．83个；每个SSR标记的多态性信息含量在0．515—0．845之间，平均为0．703。聚类分析和主成分分析结果表明，35份供试柱花草种质可分为5类，其中Ⅲ类和Ⅴ类与4个柱花草栽培品种的遗传关系较远。
To identify the genetic background and relationship of Stylosanthes varieties introduced newly and promote the further utilization of them, the genetic diversity of 31 Stylosanthes varieties introduced newly and 4 cultivars was analyzed using 23 SSR markers. Results showed that all 23 SSR markers were polymorphic among 35 Stylosanthes materials, and a total of 134 alleles were generated by these SSR markers. The number of alleles of SSR markers ranged from 3 to 11 with an average of 5.83 alleles. The polymorphic information content each SSR marker varied from 0.515 to 0.845, with an average of 0.703. Based on the results of analysis of cluster and principal components, the 35 Stylosanthes materials were divided into 5 separate clusters, and the lower genetic relation was presented between cluster Ⅲ and 4 cultivars, clusterⅤ and 4 cuhivars.
出处
《广东农业科学》
CAS
2015年第22期32-38,共7页
Guangdong Agricultural Sciences
基金
海南省自然科学基金(313067)
中央级公益性科研院所基本科研业务费专项(1630032013002)
国家现代产业体系牧草岗位科学家经费(CRAS-35)
关键词
柱花草
引进种质
SSR标记
遗传多样性
Stylosanthes spp.
introduced variety
SSR markers
genetic diversity
