不同外源激素对紫斑牡丹愈伤组织诱导的影响

Effects of different hormone combinations on callus induction of Paeonia rockii

试验以‘美髯公’‘粉荷’‘粉冠彩带’3个紫斑牡丹品种的叶片和叶柄为材料,研究不同处理时间0.1%HgCl_2处理牡丹对叶片、叶柄污染率、褐化率、存活率及死亡率的影响.并研究了6-BA和NAA组合,2,4-D和NAA组合以及2,4-D和6-BA组合分别对紫斑牡丹叶片和叶柄愈伤组织诱导、增殖以及再分化的影响.结果表明:(1)用0.1%HgCl2进行消毒处理,叶片和叶柄的最佳消毒时间分别为6、8 min.(2)3个品种的牡丹叶片在6-BA(2.0mg/L)/NAA(0.3mg/L)处理下的愈伤组织诱导率均为最高,叶柄在6-BA(2.0mg/L)/NAA(0.4mg/L)处理下的愈伤组织诱导率均为最高;‘粉冠彩带’叶片和叶柄的愈伤组织诱导率显著高于其他两个品种;同一品种之间叶柄的愈伤组织诱导率明显高于叶片的诱导率.(3)2,4-D(0.5mg/L)/NAA(1.5mg/L)组合处理中,叶片和叶柄愈伤增殖率均达到最高,且NAA质量浓度越高愈伤组织质地越好.(4)2,4-D(0.2mg/L)/6-BA(4mg/L)处理愈伤组织再分化过程中发现有少数透明状的胚状体.

Taking in vitro leaves and leafstalks of Paeonia rockii as study materials,the effects of treating with 0. 1% HgC12 in different time on the pollution rate,browning rate, survival rate and mortality of leaves and petioles were studied. In addition, the effects of 6-BA and NAA, 2,4-D and NAA as well as 2,4-D and 6-BA combinations on callus induction,proliferation and re-differentiation of leaves and petioles of Paeonia rockii were also researched. The results showed that： （1） Using 0. 1% HgC12 for disinfection treatment, the best sterilization time for leaves and petioles were 6,8 min respectively. （2） Under the treatment of 6-BA （2. 0 mg/L）/NAA （0. 3 mg/L）, the callus induction rates of leaves from three cultivars were all the highest. Under the treatment of 6-BA （2.0 mg/L）/NAA （0. 4 mg/L）,the callus induction rates of petioles from three cultivars were all the highest. The callus induction rates of leaves and petioles of ‘Fen Guan Cai Dai’ were both significantly higher than that of other two cultivars. For the same euhivar, callus induction rate of petiole was significantly higher than that of leaf. （3） Under the treatment of 2, 4-D （0. 5 mg/L）/NAA （1.5 mg/L）, callus proiiferation rates of leaves and petioles reached to the highest,and the callus texture getting better with the increasing of the NAA concentration. （4）A few transparent em-bryoid were found in the process of callus re-differentiation under the treatment of 2,4-d （0. 2 mg/L）/6-BA （4 mg/L）.