基于指纹图谱及柚皮素含量对铁皮石斛及伪品的鉴定

Discrimination of Dendrobium officinale and its counterfeits based on naringenin content determination and fingerprints technology

为研究铁皮石斛的质量评价及其真伪的鉴别方法,试验结合指纹图谱及铁皮石斛成分柚皮素的含量测定,比较铁皮石斛及其伪品的化学成分的差异性。采用HPLC法对铁皮石斛及伪品进行分析,测定铁皮石斛中柚皮素含量,并用中药色谱指纹图谱相似度评价系统软件比较指纹图谱的相似度,以及DPS统计软件进行聚类分析和主成分分析。结果表明,正品铁皮石斛指纹图谱之间有较大相似性,与伪品有较大差异性,且柚皮素含量有一定差异。建立的铁皮石斛指纹图谱技术结合柚皮素含量可为铁皮石斛真伪的鉴别提供技术支持。

A new method for the quality evaluation and discrimination of Dendrobium officinale and its counterfeits was developed by means of naringenin content determination combined with fingerprints technology. The differences of chemical compositions were compared between Dendrobium officinale and its counterfeits, which could provide a reference for quality evaluation and identification. HPLC was applied for determination of naringenin in Dendrobium officinale and its counterfeits, and the chromatographic fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM. DPS was used for cluster analysis and principal component analysis. The results showed that all of the Dendrobium officinales had high similarity and significant differences with the counterfeits. Fur- thermore, naringenin contents were different among them. In conclusion, the approach established in the present pa- per offered some technical support for the discrimination of Dendrobium officinale and its counterfeits.