不同HPVDNA检测方法在子宫颈肿瘤诊断中的应用研究与进展

Research and development on different HPV DNA detection methods in diagnosis of cervical tumor

人乳头瘤病毒(HPV)感染是子宫颈上皮内瘤变(CIN)及子宫颈癌的主要致病因素,并且因HPV型别的不同,其致病能力也有差别,而持续感染高危型HPV则是促使子宫颈癌发生的最主要因素。近年来,随着宫颈癌普查技术的不断改进和提高,越来越多的早期宫颈癌被及时发现与其中HPV DNA检测技术的发展密切相关。该文对比分析目前常用的几种方法如实时荧光聚合酶链反应(PCR)法、基因芯片法、杂交捕获法(HC-Ⅱ)、高危型人乳头状瘤病毒DNA(酶切信号放大法)(HR-HPV)检测法、Cobas HPV检测,以期更好地服务临床。

Human papilloma virus （HPV） infection is the main pathogenic factor of cervical intraepithelial neoplasia （CIN） and cervical cancer, and pathogenic ability is different due to different HPV types. But the persistent infection of high-risk HPV type is the most important factor of carcinogenesis of cervical cancer. In recent years, along with the development and improvement of cervical cancer census techniques, more and more early cervical cancer can be discovered in time partially due to the development of HPV DNA detection technology. This paper made comparative analysis of several commonly used methods, such as Real-time PCR, gene chip, Hybrid Capture Ⅱ , Cervista HR-HPV and Cobas HPV DNA detection for better service for the clinics.