摘要
利用EST结合RACE方法从象耳豆根结线虫(Meloidogyne enterolobii)中克隆一个新的果胶酸裂解酶基因Me-pel2(Gen Bank登录号KP987180)。Me-pel2 c DNA开放阅读框全长834 bp,推导编码277个氨基酸残基的蛋白,属于多糖裂解酶第III家族新成员。Me-PEL2与南方根结线虫果胶酸裂解酶Mi-PEL3氨基酸具有较高的一致性,为54%。发育表达结果显示,Me-pel2在2龄幼虫和雄虫期高丰度表达,而在固着期寄生阶段转录水平急剧下降,推测Me-pel2主要在象耳豆根结线虫迁移性阶段起重要作用,通过降解寄主细胞壁果胶质成分,协助幼虫侵入寄主以及在寄主体内迁移。
Cloning and identifying parasitism genes are the keys to understand the molecular basis on nematode parasitism of plants. In this study, a new pectate lyase gene Me-pel2 （GenBank accession no. KP987180） was cloned from the root-knot nematode Meloidogyne enterolobii by EST analysis and RACE technology. The open reading frame of Me-pel2 cDNA was 834 bp long and encoded a deduced 277 amino acid sequnce belonging to polysaccharide lyases famlily Ⅲ. The deduced protein Me-PEL2 shared high identity （54%） with pectate lyase Mi-PEL3 from M. incognita. Semiquantitive RT-PCR analysis confirmed that Me-pel2 transcripts were strongly detected in motile stages （second stage juveniles and adult males） and were weak in sedentary stages. These results indicated that Me-PEL2 may play a role in plant cell wall-degrading during the penetration and migration of M. enterolobii in plant tissues.
出处
《热带作物学报》
CSCD
北大核心
2016年第1期92-98,共7页
Chinese Journal of Tropical Crops
基金
国家自然科学基金(No.31401717)
中央级公益性科研院所基本科研业务费专项(No.20131J001)
