路易斯安那鸢尾LiNramp2基因克隆与定量表达分析

Cloning and Expression Analysis of LiNramp2 Gene from Louisiana iris

利用RACE技术克隆了路易斯安那鸢尾LiNramp2基因全长cDNA序列,其大小为1 786 bp,预测编码519个氨基酸,蛋白质具有11次跨膜结构域,与其他Nramp蛋白同源性达80%以上,说明LiNramp2同其他同源基因保守性很高。定量表达结果显示,LiNramp2在路易斯安那鸢尾叶片和雄蕊中表达量较高,在雌蕊中表达量最低,根和茎表达量差别不大。随着铅处理时间增加,LiNramp2基因在根系表现缓慢下降,在叶片中表现先升高后下降的趋势,但差异不显著。

Louisiana iris is a kind of high ornamental value of perennial plants, found to be tolerance and accumulation of heavy metal Pb. However, research on the molecular tolerance mechanism of Pb was limited. Nramp membrane proteins transport many kinds of metal ions. The intermediate fragment of LiNramp2 was obtained by analyzing the Louisiana iris transcriptome. The LiNramp2 gene was cloned by using RACE technology and its length was 1 786 bp, encoding 519 aa. The protein LiNramp2 was with 11 transmembrane domains, and the conservative analysis found that there were more than 80% similar sites with others of the same source Nramps, showing LiNramp2 was highly conservative with other homologous genes. LiNramp2 expressed more in leaf and rhizome and the lowest in pistil, and the expressions in root and rhizome were not significantly different. The gene expression of LiNramp2 in the root decreased slowly as Pb treatment time increased, in the leaf, increased first and then decreased without significance. This study will be beneficial to further studies of the relationship between LiNramp2 and Pb tolerance of Louisiana iris.