摘要
目的研究淋巴细胞相关微小核糖核酸(miRNAs)在肺炎支原体肺炎(MPP)患儿的表达差异。方法采用miRNA芯片技术筛选MMP患儿12例(A组)和非MPP患儿12例(C组)血液淋巴细胞中差异表达的miRNAs,RT-PCR方法验证芯片检测结果和检测参与淋巴细胞调节相关的特异性miRNAs表达情况。结果 A组初步建立MPP患儿血液淋巴细胞中miRNAs异常表达谱,筛选出105个上调表达的miRNAs和133个下调表达的miRNAs。与C组相比,A组30个miRNAs上调大于5倍,10个miRNAs上调大于10倍,133个miRNAs下调小于0.5倍(P<0.05)。A组miRNA-181a、miRNA-152-3p、miRNA-126-3p、miRNA-29c-3p和miRNA-20-3p表达水平均高于C组(P<0.05)。结论 miRNAs在MPP患儿血液淋巴细胞中存在差异表达,可能通过调节淋巴细胞功能参与免疫反应。
Objective To study the differential expressions of lymphocyte-related microRNAs（miRNAs）in children with mycoplasma pneumoniae pneumonia（MPP）.Methods The differential expressions of miRNAs in lymphocytes of children with MPP（group A,12 cases）and non-MPP children（group C,12 cases）were screened by miRNA chip technique.The results were verified by RT-PCR chip and the expressions of lymphocyte-related miRNAs were detected in two groups.Results Abnormal expression spectrum of miRNAs in group A was initially built,in which 105 miRNAs were upregulated and 133 miRNAs were downregulated.Compared with group C,the upregulation of 30 miRNAs in group A was more than 5 times and that of 10 miRNAs was more than 10 times,but the downregulation of 133 miRNAs was less than 0.5 times（P〈0.05）.Moreover,the expressions of miRNA-181 a,miRNA-152-3p,miRNA-126-3p,miRNA-29c-3p and miRNA-20-3p were higher in group A than those in group C（P〈0.05）.Conclusion The differential expressions of miRNAs in blood lymphocytes of MPP children exist,which participate in the immune reactions by regulating the function of lymphocytes.
出处
《江苏医药》
CAS
2016年第2期142-145,共4页
Jiangsu Medical Journal
基金
国家自然科学基金(81370132)
