山羊流产嗜衣原体广东株的鉴定及分子特征分析

Identification and molecular characteristic of Chlamydophila abortus Guangdong strain in goats

目的确诊疑似羊衣原体感染的病例,分析羊衣原体分子特征。方法从广东某羊场采集疑似羊流产嗜衣原体病例的子宫内羊水,采用现场病理解剖观察、病料触片吉姆萨染色、显微镜观察、特异性目的基因ompA扩增和序列测定及遗传进化关系分析等方法进行了该病例的确诊及病原分子特征分析。结果现场解剖发现和羊流产嗜衣原体感染症状极其吻合,吉姆萨染色后显微镜观察可见衣原体特有的特征,自病料中扩增出特异性目的基因片段,表明该病例为山羊感染流产嗜衣原体引起。基因序列遗传进化分析表明该流产嗜衣原体(GenBank登录号KP984478)与2013年公布的中国贵州分离株KF130872遗传关系最近(同源性为99.1%),属于同一进化分支,与2002年公布的德国分离株AJ004873遗传关系最远(同源性为98.2%)。结论本研究为山羊流产嗜衣原体的最新流行病学动态奠定了基础。

To identify suspected goats enzootic abortion cases and analyze the molecular characteristic of the pathogen, tissue samples were collected and detected by pathology examination, GIMSA dying assay, microscopic examination, specific gene amplification and sequencing, and the phylogenic tree based on gene ompA was performed. Results showed that the pathologic changes were similar with Chlarnydophila abortus （C. abortus） infection, the typical characters of Chlamydia infection were observed by microscope after GIMSA dying and specific gene ompA was amplified by PCR, indicating the disease was caused by C. abortus. Gene ompA sequence was submitted to GenBank and obtained accession number KP984478. Phylogenetic tree anal- ysis revealed that the C. abortus strain identified had a closest genetic relationship with KF130872 isolated from Guizhou in 2013（with 99. 1% homology）, and had the farthest relationship with AJ004873 published in 2002（with 98.2% homology）. Results of this paper laid the foundation for epidemiologic further study on C. abortus.