正交试验法优选大蝎子草总黄酮提取工艺

Optimization of Extraction Tecnique of Total Flavonoids in Girardinia diversifolia by Orthogonal Test

目的:优选大蝎子草(HGD)总黄酮最佳提取工艺。方法:以加水量(A),提取次数(B),提取时间(C)为影响因素,采用正交试验方案L9(34)进行HGD总黄酮的提取,紫外分光光度法在波长269 nm处测定吸光度,以浸膏收率、总黄酮含量为考察指标,进行综合加权评分,确定HGD总黄酮最佳提取工艺。结果:最佳水提取工艺为A2B3C3,即18倍加水量、提取3次、每次提取3 h时可获得较高的浸膏收率及总黄酮含量;加权评分方差分析结果表明:因素B及因素C对总黄酮提取量有显著影响。结论:利用正交试验设计方法对HGB总黄酮提取工艺进行优化,为该药材的深入研发提供一定的参考。

Objective： To optimize the technique to extract total flavonoids （TFA） in Girardinia diversifolia （HGD）. Methods： The amount of water （ A）, extracting frequency （ B ） and time （ C ） were used as influencing factors, extracting HGD TFA with orthogonal test L9 （34 ）. Determination of absor- bance at the wavelength of 269 nm by UV Spectrophotometry with extract yield, content of TFA as inspection indexes. Then comprehensive weighted scoring was applied to determine optimized HGD THA extraction technique. Result： The best technique was A2B3C3, added 18 times volume of water and extracted for 3 times, 3 hours each time in order to acquire relatively high extract yield and TFA con- tent. Analysis of weighted scoring variance indicated that factor B and C had significant influence on TFA extraction amount. Conclusion： Optimization of extraction technique by orthogonal test can reduce the time of experiment.