硅胶颗粒担载的C18毛细管液相色谱整体柱的评价

Silica-particle-supported C18 Polymeric Monolithic Column Forcapillary Liquid Chromatography

在内径为150μm石英毛细管内通过原位聚合的方法制备了一种硅胶颗粒担载的C18有机聚合物整体柱.依据扫描电镜图和压力对流速关系曲线,确定了整体柱的最佳制备条件.从整体固定相的微观结构、机械稳定性、渗透性、溶胀行为和交换容量等方面对整体柱进行了评价.结果表明,该整体柱具有孔径分布均匀、机械稳定性和渗透性好、抗溶胀性强和交换容量高等优点.最后,利用较短的一段整体柱快速基线分离了一个苯及其衍生物的混合物,证明了该柱具有疏水性机理,且柱效较高.

A silica-particle-supported C 18 polymeric monolithic column was prepared by in-situ polymerization in a 150 μm i. d. fused silica capillary pre-packed with 5 μm ODS particles. The optimal conditions for prepa- ration of the column were established based on SEM images and pressure-vs-flowrate curves. The prepared col- umn was evaluated in terms of morphology, mechanical stability, swelling-shrinking behavior and loading capac- ity. The results demonstrated that the prepared column has the advantages of homogeneous structure, better me- chanical stability and permeability, smaller swelling-shrinking behavior and larger loading capacity over its or- ganic polymeric and ODS-particle-packed counterparts. Finally, a short section of the column was successfully applied to the fast baseline separation of a mixture consisting of benzene and its derivatives, illustrating that the column is of hydrophobic retention mechanism and high column efficiency.