运动训练对脑梗死大鼠室管膜下区神经新生的影响

Effect of Wheel Running on Neurogenesis in Subventricular Zone of Adult Rats post Cerebral Ischemia

目的探讨大鼠脑梗死后跑笼训练对室管膜下区神经新生及神经功能恢复的影响。方法成年雄性Sprague-Dawley大鼠48只,线栓法阻塞左侧大脑中动脉建立脑梗死模型,随机分为对照组(n=24)和训练组(n=24),两组再分为3 d、7 d、14 d和21 d亚组,每个亚组6只。对照组于普通笼内饲养,不做任何针对性训练;训练组予跑笼训练每天2次,共3周。各亚组于预定时间采用神经功能损伤严重程度量表(NSS)评估神经功能,取材行Ki67免疫荧光染色。结果 14 d和21 d时,训练组Ki67阳性细胞数较对照组增多(P<0.05)。7 d后,训练组NSS评分低于对照组(P<0.05)。结论脑梗死后跑笼训练可促进大鼠室管膜下区神经新生,可能在脑梗死后神经功能恢复的过程中发挥重要作用。

Objective To observe the effect of wheel running exercise on subventricular zone neurogenesis and the neural function in rats post cerebral ischemia. Methods 48 adult male Sprague-Dawley rats were induced cortical infarcts with left middle artery occlusion and were housed in either standard （control group, n=24） or wheel running （exercise group, n=24）. They were assessed with neurological severi- ty scores （NSS）, and the expression of Ki67 was determined with immunofluorescence, 3, 7, 14 and 21 days after training. Results Com- pared with the control group, the number of Ki67-1abeled cells in subventricular significantly increased in the exercise group 14 and 21 days after ischemia （P〈0.05）, and the NSS decreased since 7 days after ischemia （P〈0.05）. Conclusion Wheel running may promote the neuro- genesis in subventricular of adult rats after cerebral infarction, which may associate with the recovery of neural function.