摘要
目的采用超高效液相色谱法(UPLC)分析人参新炮制品—黑参制作过程中人参皂苷的含量变化。方法色谱柱:Waters BEH C18色谱柱(2.10mm×50mm×1.7μm);流动相:以乙腈-水梯度洗脱;检测波长:203nm;柱温:35℃;流速:1.0m L/min;进样体积:10μL。结果在选定的色谱条件下,从黑参不同蒸制温度、不同蒸制次数的制作过程中化学成分发生了变化。结论实验中所建立的方法稳定、可靠,为黑参的标准制作工艺、质量评价控制提供了依据。
Objectives： To analyze the content changes of ginsenosides in the process of makingBlack Ginseng by UPLC. Methods： Chromatographic column was Waters BEH C18 （2. l Omm × 50mm × 1.7μm） and mobile phase was acetonitrile - water gradient elution, with 203nm of detection wavelength, at 35℃ of column temperature, in 1.0mL/min of flow rate and 10μL of sample injection volume. Re- suits： Under the selected chromatographic conditions, the chemical compositions ofBlack Ginseng changed when processed by different steamed temperatures and times. Conclusion： The method established in the experiments is stable and reliable, providing a basis for stand- ard nroduction orocess and aualitv control to makingBlack Ginseng.
出处
《云南中医中药杂志》
2016年第1期62-63,共2页
Yunnan Journal of Traditional Chinese Medicine and Materia Medica
基金
辽宁省博士启动基金项目:黑参抗肿瘤药效物质基础研究(NO:20121135)
